The study focused on the comparative determination of the effects of the combination of leaf extracts of Carica papaya CP and Vernonia amygdalina VA and Artemether based combination therapy ACTs on Plasmodium berghei infected male wistar rats. Fifty 50 male wistar rats, weighing 100 to 150g, were allotted into 5 groups n=7 . Group 2 negative control was infected but not treated, groups 3 5 which were infected received 250mg kg b.w each of CP and VA in combination and 4mg kg b.w of the two reference ACTs. Treatment was done orally once daily for 3 consecutive days, after which the animals were sacrificed and haematological analysis carried out. Afterwards, an observatory period of 7 days commenced after which another round of sacrifice was done.Before treatment, parasitemia count of animals in groups 3 5 was substantially p 0.05 higher when juxtaposed with group 2. AST and ALT activities were significantly p 0.05 elevated in group 3 5 when matched with group 2. Plasmodium berghei induction meaningfully p 0.05 lowered white blood cell WBC at all groups. After treatment, the extracts and drug which notably p 0.05 lowered plasmodium count, RBC, WBC, PCV, Hb and Platelet levels, did not meaningfully p 0.05 affect the activities of ALP, AST and ALT. After 7 days of observation, the extracts and drug efficaciously p 0.05 reduced plasmodium count, WBC and ALP activity further at all groups. These results indicate that the leaf extracts in combination are as efficacious as the ACTs. Ajeka Prisca Ojochogu | Igbokwe Ugochukwu Vincent | Okonkwo Onyeka Chukwudi | Nnyaha Anthonia. E. | Ikechukwu Samuel Nwaisaac | Okpa Precious Nwaka "Combination Therapy of Carica Papaya and Vernonia Amygdalina Leaf Extracts are as Efficacious as Acts" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46293.pdf Paper URL: https://www.ijtsrd.com/medicine/physiology/46293/combination-therapy-of-carica-papaya-and-vernonia-amygdalina-leaf-extracts-are-as-efficacious-as-acts/ajeka-prisca-ojochogu
International Journal of Trend in Scientific Research and Development (IJTSRD)
Volume 5 Issue 5, July-August 2021 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470
@ IJTSRD | Unique Paper ID – IJTSRD46293 | Volume – 5 | Issue – 5 | Jul-Aug 2021
Page 2031
Combination Therapy of Carica Papaya and Vernonia
Amygdalina Leaf Extracts are as Efficacious as Acts
Ajeka Prisca Ojochogu, Igbokwe Ugochukwu Vincent, Okonkwo Onyeka Chukwudi,
Nnyaha Anthonia. E., Ikechukwu Samuel Nwaisaac, Okpa Precious Nwaka
Department of Human Physiology, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria
ABSTRACT
The study focused on the comparative determination of the effects of
the combination of leaf extracts of Carica papaya (CP) and Vernonia
amygdalina (VA) and Artemether-based combination therapy (ACTs)
on Plasmodium berghei infected male wistar rats. Fifty (50) male
wistar rats, weighing 100 to 150g, were allotted into 5 groups (n=7).
Group 2 (negative control) was infected but not treated, groups 3-5
which were infected received 250mg/kg b.w each of CP and VA in
combination and 4mg/kg b.w of the two reference ACTs. Treatment
was done orally once daily for 3 consecutive days, after which the
animals were sacrificed and haematological analysis carried out.
Afterwards, an observatory period of 7 days commenced after which
another round of sacrifice was done.
Before treatment, parasitemia count of animals in groups 3-5 was
substantially (p<0.05) higher when juxtaposed with group 2. AST
and ALT activities were significantly (p<0.05) elevated in group 3-5
when matched with group 2. Plasmodium berghei induction
meaningfully (p<0.05) lowered white blood cell (WBC) at all groups.
After treatment, the extracts and drug which notably (p<0.05)
lowered plasmodium count, RBC, WBC, PCV, Hb and Platelet
levels, did not meaningfully(p>0.05) affect the activities of ALP,
AST and ALT. After 7 days of observation, the extracts and drug
efficaciously (p<0.05) reduced plasmodium coun