ISSN: 0973-4945; CODEN ECJHAO
E-Journal of Chemistry
http://www.e-journals.net Vol. 4, No. 4, pp. 536-545, October 2007
Analysis of Three Penicillin Antibiotics (Ampicillin,
Amoxicillin and Cloxacillin) of Several Iranian
Pharmaceutical Companies by HPLC
A. ASHNAGAR,* N. GHARIB NASERI
*School of Pharmacy, Ahwaz Jundi Shapour Univ. of Medical Sciences, Ahwaz, Iran.
Ahwaz Faculty of Petroleum Engineering, Petroleum Univ. of Technology, Iran.
Received 4 March 2007; Accepted 30 March 2007
Abstract: Penicillin has been the most widely used antibiotic for many gram-
positive bacterial infections. In this research the purity of the standard active
ingredients of the various dosage forms of three penicillins (Amoxicillin,
Cloxacillin and Ampicillin) imported and the purity percentage of the active
ingredients in each of the various dosage forms of these drugs manufactured
by several pharmaceutical companies of Iran (Kosar, Farabi and Jaber Ibn
Hayan) were investigated and determined by HPLC technique. The analyses
were made by using a Knauer (Germany) Spherimage-80, ODS, 2-5 µm C18
column with 30 cm length, and i.d. 4.5 mm. A 20 µL solution from each
individual sample and the standard solution were injected separately onto the
column of an HPLC instrument which was equipped with ECW 2000
software of Knauer, Germany. The results obtained in this research have
shown that the purity percentage of the active ingredients of the standard
powder and the various dosage forms of all the drugs used, were 100%.
Keywords: Penicillin, Amoxicillin, Cloxicillin, Ampicillin, Antibiotics.
Penicillin (sometimes abbreviated PCN) refers to a group of -lactam antibiotics used in the
treatment of bacterial infections caused by susceptible, usually gram-positive, organisms.
The name “penicillin” can also be used in reference to a specific member of the penicillin