SCI IVF Hospital
Where Life begins
Factors to Consider for Successful
Blastocyst Culture and Transfer
The blastocyst is an embryonic stage, comprising of 100 to 150 cells. It is approximately on the
□fth day after fertilisation. During this time, the embryo will normally move out from the
fallopian tube and enter the uterus. Once inside the uterus, the blastocyst will start attaching
itself to the uterine wall, and the process is called implantation.
The doctors at every IVF clinic will conduct the in-vitro fertilisation or intra-cytoplasmic sperm
injection (ICSI) immediately after the egg retrieval process. The resulting embryo will □nd a
place inside an incubator for further development. The stages of culture are here for your
1. Day 0: day of egg retrieval and ICSI or IVF will happen after egg retrieval.
2. Day 1: it is the pronuclear stage where the embryologist checks how many eggs underwent
3. Day 2: the embryo reaches two to four-cell stages.
4. Day 3: now, the embryo comprises of eight cells.
5. Day 4: and it enters the morula stage.
6. Day 5 or 6: Finally, the blastocyst stage sets in.
The blastocyst culture and transfer process will be elaborate and need constant monitoring
from competent medical professionals.
Moreabout the blastocyst
The blastocyst consists of two types of cells:
Inner cell mass that will develop into the fetus
The outer layer of cells or the trophectoderm develops into the placenta.
The embryo transfer process is ideal during Day 2 or day 3 after the collection of the egg.
However, with the advancement of technology, the IVF laboratories can now allow the culture
of the embryo for □ve to six days. So it will give the embryo a higher chance to prove its
If the embryo stops developing prior to the □fth or sixth day, there is no need for transfer. And
the embryologist can identify and choose the best embryo through natural selection for the
Advantages of culturing the b