This publishable article entitled
ASSESSMENT OF THE
is hereby submitted as one of the
requirements for graduation.
KEDAR B. KARKI
Master of Veterinary Studies
GEMERLYN G. GARCIA
Chairperson, Advisory Committee
and Department of Veterinary Science
ASSESSMENT OF THE PATHOGENICITY PROPERTY OF
FUSARIUM GRAMINEARUM 1 IN BALB/C MICE
Kedar B. Karki and Gemerlyn G. Garcia2
An experiment was conducted to investigate the immunologic property,
pathogenicity and treatment of Fusarium graminearum infection. Several groups of mice
were randomly selected for the following groups: (PC, T1 and T2 were groups of mice
that respectively received a 1:1, 1:100 and 1:100,000 fungal dilution while T3, T4, and T5
were groups of mice that respectively received the same concentration but each were
treated with Diethylamine Acetarsol (Acetylarsan). A group of mice was included as a
negative control (NC).
In vitro assays were used to examine the ability of F. graminearum to produce
enzymes, which are thought as important virulence indicators. Results revealed the
ability of the pathogen to produce collagenase and elastase. In addition, histopathological
examination indicated vascular congestion and mild triaditis of the liver. Pulmonary
congestion and lymphoid hyperplasia in the spleen were noted. The fungi were recovered
from the liver, lungs, spleen and skin of the legs of some experimental animals.
Likewise, increase in weight of the spleen doubled as early as the second week (from 49
mg to 80 mg) and progressed up to the fourth week (125 mg) where it tapered off in the
untreated group. Similar increase in the weight of the spleen was observed in the treated
group (40 mg to 64 mg) but not as great as that in the untreated group (105 mg).
Hematological findings showed a lymphocytic count of 1.83 that increased to 3.356,