The numerous applications of citric acid have increased its demand. It is of great importance to produce citric acid using adequate monosaccharides and dissacharides as they have proven to be easily catabolyzed and decomposed by Fungi when compared to polysaccharides. The superiority of sucrose to other sugars is a result of strong extracellular mycelium bound invertase of A.niger which rapidly hydrolyses sucrose at low pH. This project was undertaken to isolate, screen and produce citric acid in a submerged fermentation by four different strains of Aspergillus niger using sucrose. Aspergillus niger was isolated and characterized from garden soil. Citric acid production from Aspergillus niger in a submerged basal medium was quantitatively determined using titrimetric method. Aspergillus niger strain HUS1 ASHUS1 , Aspergillus niger strain HUS7 ASHUS7 , Aspergillus niger strain HG49 ASHG49 and Aspergillus niger strain AN8 ASAN8 showed significant accumulation of citric acid from the submerged basal medium, of which ASHUS 1 significantly a 0.05 recorded the most yield from the production after 9 days of incubation. Mbah, O. G | Okpalla, J. O | Osuala, O. J | Okoye, P. O | Alagbu, P. O "Isolation and Screening for Citric Acid Production by Aspergillus Niger Using Sucrose as a Carbon Source" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46260.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/46260/isolation-and-screening-for-citric-acid-production-by-aspergillus-niger-using-sucrose-as-a-carbon-source/mbah-o-g
International Journal of Trend in Scientific Research and Development (IJTSRD)
Volume 5 Issue 5, July-August 2021 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470
@ IJTSRD | Unique Paper ID – IJTSRD46260 | Volume – 5 | Issue – 5 | Jul-Aug 2021
Page 2315
Isolation and Screening for Citric Acid Production by
Aspergillus Niger Using Sucrose as a Carbon Source
Mbah, O. G; Okpalla, J. O; Osuala, O. J; Okoye, P. O; Alagbu, P. O
Department of Chukwuemeka Odumegwu Ojukwu University Uli, Anambra State, Nigeria
ABSTRACT
The numerous applications of citric acid have increased its demand.
It is of great importance to produce citric acid using adequate
monosaccharides and dissacharides as they have proven to be easily
catabolyzed and decomposed by Fungi when compared to
polysaccharides. The superiority of sucrose to other sugars is a result
of strong extracellular mycelium-bound invertase of A.niger which
rapidly hydrolyses sucrose at low pH. This project was undertaken to
isolate, screen and produce citric acid in a submerged fermentation
by four different strains of Aspergillus niger using sucrose.
Aspergillus niger was isolated and characterized from garden soil.
Citric acid production from Aspergillus niger in a submerged basal
medium was quantitatively determined using titrimetric method.
Aspergillus niger strain HUS1 (ASHUS1), Aspergillus niger strain
HUS7 (ASHUS7), Aspergillus niger strain HG49 (ASHG49) and
Aspergillus niger strain AN8 (ASAN8) showed significant
accumulation of citric acid from the submerged basal medium, of
which ASHUS 1 significantly (α<0.05) recorded the most yield from
the production after 9 days of incubation.
KEYWORDS: Citric acid, Fungi isolation, Identification, Molecular
characterization, Aspergillus niger, Sucrose, fermentation
How to cite this paper: Mbah, O. G |
Okpalla, J. O | Osuala, O. J | Okoye, P.
O | Alagbu, P. O "Isolation and
Screening for Citric Acid Production by
Aspergillus Niger Using Sucrose as a
Carbon
Source"
Published