Smoking-Related Genomic Signatures in Non-Small Cell Lung Cancer
Pierre P. Massion1, Yong Zou1, Heidi Chen1, Aixiang Jiang1, Peter Coulson1, Christopher I. Amos2,
Xifeng Wu2, Ignacio Wistuba3, Qingyi Wei2, Yu Shyr1 and Margaret R. Spitz2.
Division of Allergy Pulmonary and Critical Care Medicine, Department of Medicine, Vanderbilt-
Ingram Comprehensive Cancer Center and Nashville VAMC, Nashville Tennessee1, Comprehensive
Cancer Center; Department of Epidemiology2 and Pathology3, the University of Texas M. D.
Anderson Cancer Center, Houston, TX
Address of correspondence: Pierre P. Massion, MD, Division of Allergy, Pulmonary and Critical Care
Medicine, Thoracic Oncology Center, Vanderbilt-Ingram Comprehensive Cancer Center, 2220 Pierce
Avenue, 640 Preston Research Building, Nashville, TN 37232-6838. E-mail:
Subject category 85: Lung Cancer: diagnostic methods
Word count: 4217
Running Title: Smoking signature in lung cancer
Funding support: This work was supported by CA102353, lung SPORE CA90949, lung SPORE
CA70907, CA55769 and the Damon Runyon Cancer Research Foundation (Ci-# 19-03 to PPM).
This manuscript has an online data supplement which is accessible in this issue's table of content online
AJRCCM Articles in Press. Published on September 5, 2008 as doi:10.1164/rccm.200801-142OC
Copyright (C) 2008 by the American Thoracic Society.
Abstract (249 words)
Rational: Tobacco smoking is responsible for 85 % of all lung cancers. To further our understanding
of the molecular pathogenesis of lung cancer we determined whether smoking history leads to the
emergence of specific genomic alterations found in non-small cell lung cancer (NSCLC).
Methods: Seventy five NSCLCs were selected for this study from patients with current,
none or past smoking history including pack year information. Tissue sections were microdissected,
and DNA was extracted, purified and lab