NATIONAL ADVISORY COMMITTEE ON
MICROBIOLOGICAL CRITERIA FOR FOODS
Analytical Utility of Campylobacter Methodologies
Adopted September 28, 2005
Coral Gables, FL
TABLE OF CONTENTS
Purpose of the Document
Original Work Charge and Background
Response to Questions
What additional circumstances should be considered in order
for the Food Safety and Inspection Service (FSIS) to conclude that the
poultry baseline study should address more
Campylobacter species of Campylobacter jejuni and Campylobacter coli?
How can the Agricultural Research Service (ARS) method be most
successfully used for high volume analysis in the conduct of a baseline
study of Campylobacter presence and enumeration on poultry
(chicken, turkey, goose, etc.) carcasses, parts, and
ground product that may lead to a potential performance
standard or guideline for the regulated industry? What if
any modifications should be made as a result of
discussing this method in comparison with others
presented to the Committee? Please consider whether
the above described atmospheric conditions, media,
pre-enrichment, and storage media are acceptable
for the objective of this baseline study.
To utilize FSIS resources efficiently and effectively,
FSIS expects to maintain as much continuity as possible
between the current broiler rinse sampling for Salmonella
and the proposed sampling for Campylobacter spp.
What concerns regarding the Campylobacter spp.
sampling method need to be attended to in order
to properly address post-chill injured Campylobacter spp.
cells as well as viable non-culturable (coccoid) cells?
4. 	 What further subtyping methods should be performed
on confirmed cultures (restriction fragment length
polymorphism (RFLP), amplified fragment-length
polymorphism (AFLP), pulsed-field gel electrophoresis (PFGE),
ribosomal DNA sequencing, antibiotic susceptibility, etc.),
and what, if any, limitations do any