A Pulsed Finite-Difference Time-Domain (FDTD)
Method for Calculating Light Scattering from
Biological Cells Over Broad Wavelength Ranges
Rebekah Drezek, Andrew Dunn* and Rebecca Richards-Kortum
University of Texas at Austin, Biomedical Engineering Program, Austin, TX, 78712 USA
email@example.com , firstname.lastname@example.org
*Massachusetts General Hospital, Harvard Medical School, Charleston, MA, 02129 USA
Abstract: We combine the finite-difference time-domain method with
pulse response techniques in order to calculate the light scattering properties
of biological cells over a range of wavelengths simultaneously. The method
we describe can be used to compute the scattering patterns of cells
containing multiple heterogeneous organelles, providing greater geometric
flexibility than Mie theory solutions. Using a desktop computer, we
calculate the scattering patterns for common homogeneous models of
biological cells and also for more complex representations of cellular
morphology. We find that the geometry chosen significantly impacts
scattering properties, emphasizing the need for careful consideration of
appropriate theoretical models of cellular scattering and for accurate
microscopic determination of optical properties.
2000 Optical Society of America
OCIS codes: (290.0290) Scattering; (170.6510) Spectroscopy, tissue diagnostics
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