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Application of CellJet Technology for Precise “Printing” of
Adherent Mammalian Cells with no Loss in Viability.
Several different emerging technologies are highly dependent on precise, reliable and fast
distribution of viable cells in minute volumes. Among these technologies are cell and tissue engineering, live
cells microarray assays, high content analysis - just to name a few. Recently, Digilab has developed CellJet, a
dispensing system operating with nanoliter volumes designed for operation with live eukaryotic cell lines.
Here we describe a simple protocol for dispensing nanodroplets of adherent mammalian cells either
into liquid nutrient medium, or onto dry surfaces with minimal, or no losses in their viability. For
comparative purposes, distribution of the particular cells was done by using either a CellJet technology, a
peristaltic dispenser or manually. For fresh cell suspension preparation, mammalian cells at the middle of
their logarithmic phase of growth attached to the surface of a Petri dish, were washed with PBS, trypsinized
according to the recommended procedure, resuspended in a fresh growth medium and dispensed through
either CellJet, or peristaltic system into wells filled with (Figures 1), or without (Figures 2-4) growth
medium. In the latter case, the wells were filled with appropriate growth medium after 1-6 hrs allowance for
attachment of the cells to the plastic surface. Cells (live, or stained with Trypan Blue, or Propidium Iodide)
were visualized by using MIAS-2 microscopy at 2-10x magnification in either brightfield, or fluorescent
mode and eaZYX image analyzing software. We found that trypsinized adherent cells could be dispensed
directly on plastic without loss of viability (Figures 2, 4) when dispensed at 0.1 mm from the surface. Growth
capacity of cells dispensed with CellJet was comparable to the one dispensed either manually, or with
peristaltic dispenser (Figure 3).
Blue Diamonds: Peristaltic dispenser, Cell density: 10 cel