Pineapple Ananas comosus is an indispensible fruit that is cherished by many people due to its huge health benefits. It is peeled and sold in many markets and road sides for easy accessibility. The presence of bacteria in the peeled and ready to eat fruits was checked in this study. Peeled, sliced and cellophane packaged pineapple fruits were purchased from Eke Awka Market in Anambra State Nigeria. Nutrient agar was used to carry out bacterial isolation using pour plate technique. Results showed that colony count of the pineapple fruits ranged from 3.5 9.5 2cfu ml of the rinsed water. The isolates were identified on the basis of their colony and morphological features as well as biochemical and sugar fermentation tests. Gene sequencing was used to confirm the species of some of the isolates. A total of six bacteria species were isolated and identified with frequencies as Streptococcus spp 13.9 , Pseudomonas aeruginosa 22.2 , Staphylococcus aureus 25.0 , Micrococcus luteus 11.1 , Escherichia coli 19.5 and Staphylococcus epidermidis 8.3 . Staphylococcus aureus has the highest frequency followed by Pseudomonas aeruginosa. Staphylococcus epidermidis has the least frequency. Almost all the isolates are pathogenic in nature and their presence in the consumable fruits indicates possible health problems to the consumers. The presence of E. coli indicates direct or indirect fecal contamination. Proper handling of pineapple fruits, hygiene and proper storage will help reduce the risk of contamination by these organisms. Umeh S. O. | Okafor O. I. | Chidubem-Nwachinemere, N. O "Isolation and Identification of Bacteria from Peeled and Ready to Eat Pineapple (Ananas Comosus) Fruits Retailed at Eke Awka Market, Anambra State, Nigeria" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd45050.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/45050/isolation-and-identification-of-bacteria-from-peeled-and-ready-to-eat-pineapple-ananas-comosus-fruits-retailed-at-eke-awka-market-anambra-state-nigeria/umeh-s-o
International Journal of Trend in Scientific Research and Development (IJTSRD)
Volume 5 Issue 5, July-August 2021 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470
@ IJTSRD | Unique Paper ID – IJTSRD45050 | Volume – 5 | Issue – 5 | Jul-Aug 2021
Page 1237
Isolation and Identification of Bacteria from Peeled and Ready to
Eat Pineapple (Ananas Comosus) Fruits Retailed at
Eke Awka Market, Anambra State, Nigeria
Umeh S. O.; Okafor O. I.; Chidubem-Nwachinemere, N. O
Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Nigeria
ABSTRACT
Pineapple (Ananas comosus) is an indispensible fruit that is cherished
by many people due to its huge health benefits. It is peeled and sold
in many markets and road sides for easy accessibility. The presence
of bacteria in the peeled and ready to eat fruits was checked in this
study. Peeled, sliced and cellophane packaged pineapple fruits were
purchased from Eke Awka Market in Anambra State Nigeria.
Nutrient agar was used to carry out bacterial isolation using pour
plate technique. Results showed that colony count of the pineapple
fruits ranged from 3.5 - 9.5-2cfu/ml of the rinsed water. The isolates
were identified on the basis of their colony and morphological
features as well as biochemical and sugar fermentation tests. Gene
sequencing was used to confirm the species of some of the isolates. A
total of six bacteria species were isolated and identified with
frequencies as Streptococcus spp (13.9%), Pseudomonas aeruginosa
(22.2%), Staphylococcus aureus (25.0%), Micrococcus luteus
(11.1%), Escherichia coli (19.5%) and Staphylococcus epidermidis
(8.3%). Staphylococcus aureus has the highest frequency followed by
Pseudomonas aeruginosa. Staphylococcus epidermidis has the least
frequency. Almost all the isolates are pathogenic in nature and their
presence in the consumable fruits indicates possible health problems
to the consumers. The presence of E. coli indicates direct or indirect
fecal contamination. Proper handli