Urban and peri-urban agriculture can play a crucial role in the economy, social and dietary life of urban dwellers. The city of Nairobi is going through urbanization challenges including the provision of food, especially in slum areas where the majority of urban poor live. Reliable data on extent of urban/peri-urban areas being used for farming in Nairobi County, the spatial distribution of such areas, type of crops, animals, and proximity to market places are lacking. The aim of this study was to determine the occurrence level of farming activities across four major network links of Nairobi County. Purposive sampling was applied in selecting four major network links (road transects). Semi-structured questionnaires were administered to farmers through face-to-face interviews. Data was collected on socio-economic characteristics, agricultural enterprises, and consumption patterns. Data were analyzed at a 5% level of significance, using descriptive statistics and a chi-square test was performed. Farming activities generally increased with an increase in distance from the city center towards the Nairobi outskirts along three road transects. Crops only (90%) were the main farming enterprise practiced. More occurrences of farming activities were on Mombasa (33%) followed closely by Ngong (26%) and Thika superhighway (25%) road transects. The least farming activities were observed on the Waiyaki way transect (16%). Fruits (27%) and vegetables (19%) topped the crop produce grown while poultry (38.5%) and cattle (34.6%) were the most reared livestock. These research findings will supplement Nairobi City planners’ decision-making process concerning urban and peri-urban agriculture in regard to land use allocation.