Bioresource Technology 100 (2009) 878–882
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Bioresource Technology
journal homepage: www.elsevier .com/locate/bior tech
Ethanol fermentation in a magnetically fluidized bed reactor with immobilized
Saccharomyces cerevisiae in magnetic particles
Chun-Zhao Liu a,b,*, Feng Wang a,b, Fan Ou-Yang a
aNational Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 1 Zhongguancun bei-er-tiao, Beijing 100190, PR China
bGraduate School of Chinese Academy of Sciences, Beijing 100049, PR China
a r t i c l e
i n f o
Article history:
Received 11 May 2008
Received in revised form 2 July 2008
Accepted 12 July 2008
Available online 28 August 2008
Keywords:
Ethanol fermentation
Immobilized cell
Magnetically stabilized fluidized bed reactor
Molasses
Saccharomyces cerevisiae
0960-8524/$ - see front matter 2008 Elsevier Ltd. A
doi:10.1016/j.biortech.2008.07.016
* Corresponding author. Address: National Key Lab
neering, Institute of Process Engineering, Chinese Ac
guancun bei-er-tiao, Beijing 100190, PR China. Tel./fa
E-mail address: czliu@home.ipe.ac.cn (C.-Z. Liu).
a b s t r a c t
Ethanol fermentation by immobilized Saccharomyces cerevisiae cells in magnetic particles was success-
fully carried out in a magnetically stabilized fluidized bed reactor (MSFBR). These immobilized magnetic
particles solidified in a 2 % CaCl2 solution were stable and had high ethanol fermentation activity. The
performance of ethanol fermentation of glucose in the MSFBR was affected by initial particle loading rate,
feed sugar concentration and dilution rate. The ethanol theoretical yield, productivity and concentration
reached 95.3%, 26.7 g/L h and 66 g/L, respectively, at a particle loading rate of 41% and a feed dilution rate
of 0.4 h1 with a glucose concentration of 150 g/L when the magnetic field intensity was kept in the range
of 85–120 Oe. In order to use this developed MSFBR system for ethanol production from cheap raw mate-
rials, cane molasses was used