CERTIFICATE OF ANALYSIS
10X Buffer Tango™
(with BSA)
#BY5
5 x 1 ml
Lot:
Store at -20°C
BSA included
Description
Tango™ buffer, being a part of Five Buffer System, is
specially designed for use in double digestions.
For more information see Fermentas Catalog or
www.fermentas.com/doubledigest.
1X Buffer Composition
33 mM Tris-acetate (pH 7.9 at 37°C),
10 mM magnesium acetate,
66 mM potassium acetate,
0.1 mg/ml BSA.
QUALITY CONTROL
Functional test
Functionaly tested in DNA digestion with 15 randomly
picked conventional restriction endonucleases.
Digestion conditions are as described in activity
assays of each enzyme. Reaction products analyzed
by agarose electrophoresis and ethidium bromide
staining. No change in digestion pattern is observed
compared to previous lot.
Endo- and Exonucleases Assay
Single stranded and double standed radiolabeled
oligonucleotides are incubated with the 1X buffer at
37°C for 16 hours, separated on polyacrylamide gel
and analyzed by phosphoimaging. No signs of
radiolabeled oligo degradation is considered as the
absence of endo- and exonucleases activities.
Nicking activities Assay
Supercoiled pUC19 DNA is incubated with the 1X
buffer at 37°C for 17 hours and analyzed by agarose
gel electrophoresis and ethidium bromide staining.
There is no linearised plasmid form and relaxation of
supercoiled plasmid form compared to negative
control.
Quality authorized by:
Jurgita Zilinskiene
Important Note
This buffer contains BSA. In order to avoid the risk of
bacterial growth, do not store buffer containing BSA
at 4°C for periods longer than one week. If buffer is to
be stored at 4°C it should be aliquoted into small
portions and the main stock should be kept at -20°C.
PRODUCT USE LIMITATION.
This product is developed, designed and sold exclusively for research purposes and
in vitro use only. The product was not tested for use in diagnostics or for d