www.mercodia.com
Mercodia Equine Insulin ELISA
10-1205-01
n Optimized for equine samples
n Monoclonal antibodies
n High precision
n Easy-to-use format
n Liquid calibrators
n Assay range: 0.02– 1.5 µg/l
n Sample volume: 25 µl
n Incubations (min): 120 min + 15 min
n Samples: Serum, plasma or culture medium
A high quality enzyme immunoassay for the quantifi cation of
equine insulin.
Summary
Insulin is the principal hormone responsible for the control of
glucose metabolism. It is synthesized in the ß-cells of the islets
of Langerhans as the precursor, proinsulin, which is processed
to form C-peptide and insulin. Both are secreted in equimolar
amounts into the portal circulation. The mature insulin mol-
ecule comprises two polypeptide chains, the A chain and the B
chain. The two chains are linked together by two inter-chain di-
sulphide bridges. There is also an intra-chain disulphide bridge
in the A chain.
Secretion of insulin is mainly controlled by plasma glucose
concentration, and the hormone has a number of important
metabolic actions. Its principal function is to control the uptake
and utilisation of glucose in peripheral tissues via the glucose
transporter. This and other hypoglycaemic activities, such as the
inhibition of hepatic gluconeogenesis and glycogenolysis are
counteracted by the hyperglycaemic hormones including glu-
cagon, epinephrine (adrenaline), growth hormone and cortisol.
Matching energy intake with the energy expenditure is impor-
tant for the exercising horse. Parameters such as the hormones
ghrelin, adiponectin and insulin have shown to play a major
role in mediating the energy balance either through their ef-
fects on feed intake or metabolic regulation. Glucose and insu-
lin responses are modifi ed by the diet and obesity, exercise level
and stress also alters glucose and insulin metabolism.
2008-02-20, Version 2, Article No 32-4142
Order No:
10-1205-01
1x96 wells
Test principle
Mercodia Equine Insulin ELISA is a solid phase two-site en-
zyme immunoassay based on th