126
Original Article
J. Clin. Biochem. Nutr., 43, 126–128, September 2008
JCBN
ournal of Clinical Biochemistry and Nutrition
0912-0009
1880586
the Society for Free Radical Reserch Japan
Kyot, Japan
jcbn2
057
0.3164/jcb.2008057
Original Article
Eicosapentaenoic Acid Suppresses the Proliferation of Synoviocytes
from Rheumatoid Arthritis
Masahide Hamaguchi1, Yutaka Kawahito1,*, Atsushi Omoto1, Yasunori Tsubouchi1,
Masataka Kohno1, Takahiro Seno1, Masatoshi Kadoya1, Aihiro Yamamoto1,
Hidetaka Ishino1, Masahide Matsuyama2, Rikio Yoshimura2, and Toshikazu Yoshikawa1
1Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine,
Kamigyo-ku, Kyoto 602-8566, Japan
2Department of Urology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan
92008
143268
Received 25.4.2008
; acepted 30.4.2008
*To whom correspondence should be addressed.
Tel: +81-75-251-5505 Fax: +81-75-252-3721
E-mail: kawahity@koto.kpu-m.ac.jp
Received 25 April, 2008; Accepted 30 April, 2008
Copyright © 2008 JCBN
Summary Eicosapentaenoic acid (EPA) is essential for normal cell growth, and may play an
important role in inflammatory and autoimmune disorders including rheumatoid arthritis. We
investigate that EPA could suppress the proliferation of fibroblast like synoviocytes in vitro.
We treated synoviocytes with 1 to 50 µM EPA and measured cell viabilities by the modified
MTT assay. We sorted the number of them in sub G1 stage by fluorescence-activated cell
sorting caliber. And we stained them by light green or Hoechst 33258, and investigate micro-
scopic appearance. The cell viabilities were decreased at 30 µM, 40 µM, and 50 µM of EPA
comparing to 0 µM of EPA. The half maximal concentration of synoviocytes inhibition was
approximately 25 µM. At day 1 and day 3, cell number was also decreased at 50 µM EPA com-
paring to control. FACS caliber indicated the number of synoviocytes in sub G1 stage did not
increase in each concentration of EPA. Hoechst staining indicated normal chro