Bioinformatic Evaluation of a Sequence for Custom
TaqMan® Gene Expression Assays
The Custom TaqMan® Gene Expression Assays are custom assays that are designed,
synthesized, formulated, and delivered as analytically quality-controlled primer and
probe sets for gene expression assays based on sequence information submitted by the
customer. The goal of this tutorial is to help the researcher evaluate the quality of their
sequence information before submitting an order for a Custom TaqMan® Gene
Expression Assay. Specific information is given on how to assess a sequence using a
variety of on-line tools.
The Custom TaqMan® Gene Expression Assays provide the researcher the opportunity
to design an assay that is not currently available through the TaqMan® Gene Expression
Assays offerings. Studies that involve viral detection, species other than human, mouse,
rat, Arabidopsis, Drosophila, C. elegans, canine or Rhesus macaque, or detection of
specific pathogens are some examples of applications that would benefit from this
custom design line of products. For gene expression assays of the species mentioned
above, the TaqMan® Gene Expression Assays should be used. If a particular gene
target is currently not available then one should consider a custom design.
Note: Additional TaqMan® Assays are regularly added to the web site for ordering. Please visit
the TaqMan® Gene Expression Assays Search page for regular updates. To learn more about
how to order assays, please download the Online Ordering Guide for TaqMan® Gene Expression
Ordering Custom TaqMan® Assays involves the following procedures:
1. Selecting a target sequence
2. Assessing the quality of the sequence
3. Preparing the submission file using the Custom TaqMan® Genomic Assays
File Builder software
4. Formatting the sequence for submission
5. Submitting the order via the File Builder software or e-mail.
Step two, Assessing the quality of the sequence, will be covered in this tutorial.