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Fluorescence Labeling of Antibody
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Our custom fluorescent antibody labeling services combine years of conjugation expertise with a wide range of labeling options, including fluorescent dyes,
phycobiliproteins and enzymes, as well as the selection of labeled chemicals. We provide conjugate services for research and commercial purposes.
Immunofluorescence is a technique for fluorescent labeling of specific biological targets in samples using antibodies. Antibody is a Y-type high molecular
glycoprotein, also known as immunoglobulin, which can bind specifically to other molecules (often referred to as antigens or epitopes). Fluorescent antibody
labeling technique is to combine fluorescein with specific antibody by chemical method to form fluorescein-protein conjugate (fluorescent labeled antibody),
which still retains antibody activity and has the tracer effect of fluorescein. When it was specifically bound to the corresponding antigen, it showed bright specific
fluorescence with the help of fluorescence microscope.
Isothiocyanyl fluorescent yellow (FITC) is commonly used as a marker in the experiment. In alkaline solution, the chemical group isothiocyanate (- N=C=S) on
FITC binds to the free amino group of antibody protein (mainly the ε-amino group of lysine). Fluorescent antibodies were formed to determine the presence of
the corresponding antigens in the cells.
Immunocytochemical (ICC) usually uses fluorescent labeled antibodies to study the subcellular distribution of proteins.
Cell permeable living cell dye, used in cell viability, ion, pH, nitric oxide, oxygen and stem cell culture.
Deep knowledge and rich experience in biomaterial modification and conjugat