M.SC., BIOCHEMISTRY I SEMESTER
BC: 1.1: Chemistry of Biomolecules
Unit – 1: Amino acids – classification, structure and physiochemical properties, chemical synthesis of
peptides – solid phase peptide synthesis. Proteins – classification, purification, and criteria of
homogeneneity. Structural organization, sequence determination and characterization of proteins.
Conformation of proteins – Ramachandran plots. Denaturation of proteins.
Unit – 2 :Classification, chemical properties of carbohydrates, Chemistry and biological roles of homo
and heteropolysaccharides, peptidoglycan, glycosaminoglycans, glycoconjugates, glycoproteins,
Structural elucidation of polysaccharides; Oligosaccharides – lectin interaction in biochemical
Unit – 3: Classification of Lipids, Fatty acids and their physiochemical properties. Structure and
properties of Prostaglandins. Fats and waces, physicochemical properties and characterization of fats
and oil. Structure, properties and biological roles of phospholipids and Sphingolipids. Chemistry and
properties of Sterols and Steroids. Salient features of bacterial and plant lipids.
Unit – 4: Nucleic acids – bases, nucleosides, nucleotides, physicochemical properties of nucleic
acids, cleavage of nucleic acids by enzymatic methods, non – enzymatic transformation of
nucleotides and nucleic acids, methylation, Sequencing, chemical synthesis of DNA. Three
dimensional structure of DNA. Different froms of DNA – circular DNA and Supercoiling. Types of RNA.
Strucure of t-RNA. Nucleotids as regulatory molecules, enzyme fofactors and mediatros of chemical
energy in cells. Pporphyrins – Structure and properties of porphyrins – heme , Chlorophyll and
BC 1.2: Biochemical Techniques
Unit – 1: Separation Techniques: Principles, methods and applications of chromatography – Paper,
ion exchange, ge