Pullulanase is an extracellular carbohydrase responsible for the hydrolysis of pullulan and amylopectin toproduce maltotriose. The product maltotriose is used in detergent industry, bakery industry and in the production of biotechnological products. In the present investigation pullulanase producing bacillus species were isolated and characterized using different biochemical and molecular methodologies. The isolates were identified as Bacillus cereus and Bacillus thuringiensis respectively.. The pullulanase acivity was higher in Bacillus cereus, 0.62U ml than B. thuringiensis, 0.53 U ml. This research reveals that pullulanase enzyme production from these Bacillus species shows great promise for use in industrial processes. Nwozor, N. C | Ogbo, F. C "Isolation and Molecular Characterization of Pullulanase Producing Bacillus Strains" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd45051.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/45051/isolation-and-molecular-characterization-of-pullulanase-producing-bacillus-strains/nwozor-n-c
International Journal of Trend in Scientific Research and Development (IJTSRD)
Volume 5 Issue 5, July-August 2021 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470
@ IJTSRD | Unique Paper ID – IJTSRD45051 | Volume – 5 | Issue – 5 | Jul-Aug 2021
Page 1243
Isolation and Molecular Characterization of
Pullulanase Producing Bacillus Strains
Nwozor, N. C; Ogbo, F. C
Department of Appalled Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Nigeria
ABSTRACT
Pullulanase is an extracellular carbohydrase responsible for the
hydrolysis of pullulan and amylopectin toproduce maltotriose. The
product maltotriose is used in detergent industry, bakery industry and
in the production of biotechnological products. In the present
investigation pullulanase producing bacillus species were isolated
and characterized using different biochemical and molecular
methodologies. The isolates were identified as Bacillus cereus and
Bacillus thuringiensis respectively. The pullulanase acivity was
higher in Bacillus cereus, 0.62U/ml than B. thuringiensis, 0.53U/ml.
This research reveals that pullulanase enzyme production from these
Bacillus species shows great promise for use in industrial processes.
KEYWORDS: Pullulanase, Bacillus, Molecular, Characterization
How to cite this paper: Nwozor, N. C |
Ogbo, F. C "Isolation and Molecular
Characterization
of
Pullulanase
Producing Bacillus Strains" Published in
International
Journal of Trend in
Scientific Research
and Development
(ijtsrd), ISSN: 2456-
6470, Volume-5 |
Issue-5,
August
2021,
pp.1243-
1248,
URL:
www.ijtsrd.com/papers/ijtsrd45051.pdf
Copyright © 2021 by author (s) and
International Journal of Trend in
Scientific Research and Development
Journal. This is an
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distributed under the
terms of
the Creative Commons
Attribution License (CC BY 4.0)
(http://creativecommons.org/licenses/by/4.0)
INTRODUCTION
Pullulanase is an extracellular carbohydrase which
debranches pullul