CONTENTS
UNITED STATES DEPARTMENT OF AGRICULTURE
ANIMAL AND PLANT HEALTH INSPECTION SERVICE
PLANT PROTECTION AND QUARANTINE
CONTAINMENT GUIDELINES
For Educational Displays of Adult, Nonindigenous,
Butterflies and Moths (Lepidoptera)
(Revised 7/23/02)
UNITED STATES DEPARTMENT OF AGRICULTURE
ANIMAL AND PLANT HEALTH INSPECTION SERVICE
PLANT PROTECTION AND QUARANTINE
PEST PERMIT EVALUATIONS, UNIT 133
4700 RIVER ROAD
RIVERDALE, MARYLAND 20737
I. PURPOSE OF THIS DOCUMENT
II. CONSTRUCTION STANDARDS FOR THE ENTIRE STRUCTURE
Location and Identification
Floor Plan
Walls, Ceilings, and Floors
Windows
Doors
HVAC System
Electrical System
Plumbing System
III. CONSTRUCTION STANDARDS FOR SPECIALIZED ROOMS
Glasshouse
Public Display Room
Receiving Room
Vestibules
Restrooms
IV. EQUIPMENT STANDARDS
Benches, Tables and Other Furniture
Sterilization or Decontamination Equipment
Cages, Containers and Chrysalis Emergence Cabinet
V. OPERATIONAL STANDARDS
Containment Director
Authorized Personnel
Visitors
Apparel
Personal Cleanliness
Cleaning and Disinfecting the Facility
Opening and Handling Packages
Pupae
Landscaping the Display Area
PPQ Regulatory Requirements
I. PURPOSE OF THIS DOCUMENT
These guidelines are a reference to help you prepare to comply with the USDA APHIS
regulations that oversee import, interstate transport, and possession of organisms
regulated by the Plant Protection Act of 2000. The guidelines will help in designing,
building, maintaining, and operating a permanent containment facility for non-
indigenous, known species of butterflies and moths (Lepidoptera) from pure cultures.
These organisms are usually imported only as pupae. The resulting adults are typically
displayed in free-flight enclosures accessible to the public. They may be used in
educational displays in zoos, museums, and other public facilit