EN5146 COVER SHEET Kit 89856 includes Kit 89856X and Kit 89856Y Kit 89856X is stored at -20ºC Kit 89856Y is stored at +4ºC 89856X EZ-Detect Rac-1 Component # Description 89856D Anti-Racl mouse Antibody 89856E GST-Pak1-PBD 89856F 100X GDP 89856G 100X GTPyS 89856Y EZ-Detect Rac-1 Component # Description 89856A SwellGel® Immobil Glutathione 89856B 2X SDS Buffer 89856C Lysis/Binding/Wash Buffer Pierce PO BOX 117 (815) 968-0747 www.thermo.com 3747 N. Meridian Road Rockford, IlL 61105 (815) 968-7316 fax SwellGel® Immobilized Glutathione Discs 4044 89856A 2/1/2008. 2/1/2008. Material Safety Data Sheet Product name Manufacturer Product No. MSDS # Validation date Print date In case of emergency Product and company identification Responsible name MSDS (Regulatory Affairs) SwellGel® Immobilized Glutathione Discs : : : : : : : 1 . CHEMTREC: 800.424.9300 OUTSIDE US: 202.483.7616 Supplier : : Use of Substance/Preparation Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. : ThermoFisher Scientific (Milwaukee) LLC 2202 N. Bartlett Ave. Milwaukee, WI 53202 USA 414.227.3600 or 877.886.7629 ThermoFisher Scientific (Milwaukee) LLC 2202 N. Bartlett Ave. Milwaukee, WI 53202 USA 414.227.3600 or 877.886.7629 Ingestion. Emergency overview Hazards identification Routes of entry Potential acute health effects No known significant effects or critical hazards. No known significant effects or critical hazards. No known significant effects or critical hazards. No known significant effects or critical hazards. Eyes Skin Inhalation Ingestion Physical state Solid. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. Avoid exposure - obtain special instructions before use. Contains material that can cause target organ damage. : : : : : : : OSHA/HCS status : This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). 2 . Potential chronic health effects Carcinogenicity : No known significant effects or critical hazards. Mutagenicity : No known significant effects or critical hazards. Teratogenicity : No known significant effects or critical hazards. Developmental effects : No known significant effects or critical hazards. Chronic effects : Contains material that can cause target organ damage. Fertility effects : No known significant effects or critical hazards. Over-exposure signs/symptoms Ingestion : No specific data. Inhalation : No specific data. Target organs : Contains material which causes damage to the following organs: upper respiratory tract, eye, lens or cornea. 1/7 2/1/2008. SwellGel® Immobilized Glutathione Discs Hazards identification 2 . See toxicological information (section 11) Medical conditions aggravated by over- exposure Pre-existing disorders involving any target organs mentioned in this MSDS as being at risk may be aggravated by over-exposure to this product. : Skin : No specific data. Eyes : No specific data. The preparation is not classified as dangerous according to Directive 1999/45/EC and its amendments. Classification Not classified. : Trade Secret #4 1 - 3 Composition/information on ingredients Name CAS number % United States 3 . There are no ingredients or additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section. Substance/preparation Preparation : Ingredient name CAS number % Classification EC number Trade Secret #4 1 - 3 Not classified. [2] Europe [1] Substance classified with a health or environmental hazard [2] Substance with a workplace exposure limit Occupational exposure limits, if available, are listed in section 8. Wash out mouth with water. Remove dentures if any. Move exposed person to fresh air. Keep person warm and at rest. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention. Never give anything by mouth to an unconscious person. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs. Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Continue to rinse for at least 10 minutes. Get medical attention if symptoms occur. Wash clothing before reuse. Clean shoes thoroughly before reuse. Move exposed person to fresh air. Keep person warm and at rest. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. It may be dangerous to the person providing aid to give mouth-to- mouth resuscitation. Get medical attention. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. First aid measures Eye contact Skin contact Inhalation Ingestion : : : : 4 . Protection of first-aiders : No action shall be taken involving any personal risk or without suitable training. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. See section 11 for more detailed information on health effects and symptoms. 2/7 2/1/2008. SwellGel® Immobilized Glutathione Discs Use an extinguishing agent suitable for the surrounding fire. Fire-fighting measures Extinguishing media Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training. No specific fire or explosion hazard. Special protective equipment for fire-fighters Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode. : 5 . Special exposure hazards : None known. Suitable : Not suitable : Hazardous combustion products : Decomposition products may include the following materials: carbon oxides Flammability of the product : No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see section 8). Move containers from spill area. Approach release from upwind. Prevent entry into sewers, water courses, basements or confined areas. Vacuum or sweep up material and place in a designated, labeled waste container. Dispose of via a licensed waste disposal contractor. Note: see section 1 for emergency contact information and section 13 for waste disposal. Environmental precautions Accidental release measures : Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Personal precautions : Large spill : 6 . Move containers from spill area. Vacuum or sweep up material and place in a designated, labeled waste container. Dispose of via a licensed waste disposal contractor. Small spill : Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Put on appropriate personal protective equipment (see section 8). Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Do not get in eyes or on skin or clothing. Do not ingest. If during normal use the material presents a respiratory hazard, use only with adequate ventilation or wear appropriate respirator. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container. Handling and storage Handling Storage : : 7 . 3/7 2/1/2008. SwellGel® Immobilized Glutathione Discs Trade Secret #4 ACGIH (United States). TWA: 10 mg/m³ NIOSH (United States). TWA: 5 mg/m³ ACGIH TLV (United States, 1/2007). TWA: 10 mg/m³ 8 hour(s). NIOSH REL (United States, 12/2001). TWA: 5 mg/m³ 10 hour(s). Form: Respirable fraction TWA: 10 mg/m³ 10 hour(s). Form: Total OSHA PEL (United States, 11/2006). TWA: 5 mg/m³ 8 hour(s). Form: Respirable fraction TWA: 15 mg/m³ 8 hour(s). Form: Total dust OSHA PEL 1989 (United States, 3/1989). TWA: 5 mg/m³ 8 hour(s). Form: Respirable fraction TWA: 15 mg/m³ 8 hour(s). Form: Total dust OSHA PEL (United States). Notes: Respirable TWA: 15 mg/m³ 8 hour(s). Exposure controls/personal protection Product name Exposure limits Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator. Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists, gases or dusts. Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Personal protection Eyes Skin Respiratory Consult local authorities for acceptable exposure limits. : : : Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Hands : United States 8 . Engineering measures : If user operations generate dust, fumes, gas, vapor or mist, use process enclosures, local exhaust ventilation or other engineering controls to keep worker exposure to airborne contaminants below any recommended or statutory limits. Hygiene measures : Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. Recommended monitoring procedures : If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Environmental exposure controls : Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. Europe Trade Secret #4 ACGIH (United States). TWA: 10 mg/m³ ACGIH TLV (United States, 1/2007). TWA: 10 mg/m³ 8 hour(s). 4/7 2/1/2008. SwellGel® Immobilized Glutathione Discs Solid. Easily soluble in the following materials: cold water and hot water. Colorless. [Light] Physical and chemical properties Physical state Solubility Color : : : 9 . The product is stable. Under normal conditions of storage and use, hazardous polymerization will not occur. No specific data. Will not occur. Under normal conditions of storage and use, hazardous decomposition products should not be produced. No specific data. Stability and reactivity Stability Conditions to avoid Materials to avoid Hazardous decomposition products Hazardous polymerization : : : : : 10 . Toxicological information United States 11 . Acute toxicity Trade Secret #4 LD50 Oral Rat 29700 mg/kg - Product/ingredient name Result Species Dose Exposure Conclusion/Summary : Not available. Chronic toxicity Conclusion/Summary : Not available. Carcinogenicity Conclusion/Summary : Not available. Mutagenicity Conclusion/Summary : Not available. Teratogenicity Conclusion/Summary : Not available. Reproductive toxicity Conclusion/Summary : Not available. Classification Trade Secret #4 A4 - - None. - - Product/ingredient name ACGIH EPA NIOSH NTP IARC OSHA No known significant effects or critical hazards. Chronic effects : No known significant effects or critical hazards. Carcinogenicity : No known significant effects or critical hazards. Mutagenicity No known significant effects or critical hazards. Teratogenicity Developmental effects : No known significant effects or critical hazards. Fertility effects : No known significant effects or critical hazards. : : Europe 5/7 2/1/2008. SwellGel® Immobilized Glutathione Discs Ecological information United States Environmental effects : No known significant effects or critical hazards. 12 . Aquatic ecotoxicity Conclusion/Summary : Not available. Other adverse effects : No known significant effects or critical hazards. The generation of waste should be avoided or minimized wherever possible. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Waste disposal Disposal considerations Disposal should be in accordance with applicable regional, national and local laws and regulations. : 13 . Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees. Hazardous waste : Within the present knowledge of the supplier, this product is not regarded as hazardous waste, as defined by EU Directive 91/689/EEC. Transport information - DOT Classification - - - Not regulated. IATA-DGR Class - Regulatory information UN number Proper shipping name Classes PG* - Not regulated. 14 . PG* : Packing group Target organ effects HCS Classification Regulatory information U.S. Federal regulations United States inventory (TSCA 8b): Not determined. Clean Water Act (CWA) 307: No products were found. Clean Water Act (CWA) 311: No products were found. Clean Air Act (CAA) 112 accidental release prevention: No products were found. Clean Air Act (CAA) 112 regulated flammable substances: No products were found. Clean Air Act (CAA) 112 regulated toxic substances: No products were found. WHMIS (Canada) Not controlled under WHMIS (Canada). SARA 302/304/311/312 extremely hazardous substances: No products were found. SARA 302/304 emergency planning and notification: No products were found. SARA 302/304/311/312 hazardous chemicals: Trade Secret #4 SARA 311/312 MSDS distribution - chemical inventory - hazard identification: Trade Secret #4: Delayed (chronic) health hazard : : : Canada United States 15 . United States inventory (TSCA 8b) : United States inventory (TSCA 8b): Not determined. 6/7 2/1/2008. SwellGel® Immobilized Glutathione Discs Regulatory information 15 . CEPA Toxic substances: None of the components are listed. Canadian ARET: None of the components are listed. Canadian NPRI: None of the components are listed. Alberta Designated Substances: None of the components are listed. Ontario Designated Substances: None of the components are listed. Quebec Designated Substances: None of the components are listed. Australia inventory (AICS): Not determined. China inventory (IECSC): Not determined. Korea inventory (KECI): Not determined. Philippines inventory (PICCS): Not determined. Japan inventory (ENCS): Not determined. International regulations International lists : EU regulations Canadian lists : Canada inventory : Canada inventory: Not determined. This product is not classified according to EU legislation. Risk phrases : : Other information Hazardous Material Information System (U.S.A.) 1 0 0 0 0 0 National Fire Protection Association (U.S.A.) Health Special Instability Flammability Health Flammability Physical hazards CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. Label requirements : Notice to reader To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist. Date of printing Date of issue Date of previous issue Version 2/1/2008. 2/1/2008. 1 No previous validation. : : : : : : 16 . The customer is responsible for determining the PPE code for this material. Indicates information that has changed from previously issued version. 7/7 2/1/2008. 2X SDS Sample Buffer 4045 89856B 2/1/2008. 2/1/2008. Material Safety Data Sheet Product name Manufacturer Product No. MSDS # Validation date Print date In case of emergency Product and company identification Responsible name MSDS (Regulatory Affairs) 2X SDS Sample Buffer : : : : : : : 1 . CHEMTREC: 800.424.9300 OUTSIDE US: 202.483.7616 Supplier : : Use of Substance/Preparation Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. : ThermoFisher Scientific (Milwaukee) LLC 2202 N. Bartlett Ave. Milwaukee, WI 53202 USA 414.227.3600 or 877.886.7629 ThermoFisher Scientific (Milwaukee) LLC 2202 N. Bartlett Ave. Milwaukee, WI 53202 USA 414.227.3600 or 877.886.7629 Emergency overview Hazards identification Potential acute health effects Irritating to respiratory system. Exposure to decomposition products may cause a health hazard. Serious effects may be delayed following exposure. Irritating to eyes. Harmful if swallowed. Harmful in contact with skin. Irritating to skin. Eyes Skin Inhalation Ingestion Physical state Liquid. WARNING ! CAUSES RESPIRATORY TRACT, EYE AND SKIN IRRITATION. MAY BE HARMFUL IF ABSORBED THROUGH SKIN OR IF SWALLOWED. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. Harmful in contact with skin and if swallowed. Irritating to eyes, respiratory system and skin. Avoid exposure - obtain special instructions before use. Do not breathe vapor or mist. Do not ingest. Do not get in eyes. Avoid contact with skin and clothing. Contains material that can cause target organ damage. Use only with adequate ventilation. Keep container tightly closed and sealed until ready for use. Wash thoroughly after handling. : : : : : : Odor : Odorless. OSHA/HCS status : This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). 2 . Potential chronic health effects Carcinogenicity : No known significant effects or critical hazards. Mutagenicity : No known significant effects or critical hazards. Teratogenicity : No known significant effects or critical hazards. Developmental effects : No known significant effects or critical hazards. Chronic effects : Contains material that can cause target organ damage. Fertility effects : No known significant effects or critical hazards. 1/8 2/1/2008. 2X SDS Sample Buffer Hazards identification 2 . See toxicological information (section 11) Medical conditions aggravated by over- exposure Pre-existing skin and digestive disorders and disorders involving any other target organs mentioned in this MSDS as being at risk may be aggravated by over-exposure to this product. : Over-exposure signs/symptoms Ingestion : No specific data. Skin : Adverse symptoms may include the following: irritation redness Eyes : Adverse symptoms may include the following: pain or irritation watering redness Inhalation : Adverse symptoms may include the following: respiratory tract irritation coughing Target organs : Contains material which causes damage to the following organs: kidneys, lungs, mucous membranes, gastrointestinal tract, upper respiratory tract, skin, eye, lens or cornea. The preparation is not classified as dangerous according to Directive 1999/45/EC and its amendments. Classification Not classified. : sodium dodecyl sulfate 151-21-3 3 - 5 GLYCEROL; GLYCERIN; GLYCERIN, ANHYDROUS; GLYCERIN, SYNTHETIC; GLYCERINE; GLYCYL ALCOHOL; 1,2,3-PROPANETRIOL; SYNTHETIC GLYCERIN; 90 TECHNICAL GLYCERIN; TRIHYDROXYPROPANE; 1,2,3- TRIHYDROXYPROPANE; GLYGERINE MIST 56-81-5 1 - 3 1,3-propanediol, 2-amino-2-(hydroxymethyl)- 77-86-1 1 - 3 Composition/information on ingredients Name CAS number % United States 3 . There are no ingredients or additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section. Substance/preparation Preparation : Ingredient name CAS number % Classification EC number sodium dodecyl sulfate 151-21-3 3 - 5 205-788-1 F; R11 Xn; R21/22 Xi; R36/37/38 [1] Glycerol 56-81-5 1 - 3 200-289-5 Xi; R36/37/38 [1] [2] 1,3-propanediol, 2-amino-2-(hydroxymethyl)- 77-86-1 1 - 3 201-064-4 Xn; R20/21/22 [1] Europe [1] Substance classified with a health or environmental hazard [2] Substance with a workplace exposure limit Occupational exposure limits, if available, are listed in section 8. 2/8 2/1/2008. 2X SDS Sample Buffer Wash out mouth with water. Remove dentures if any. Move exposed person to fresh air. Keep person warm and at rest. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention. Never give anything by mouth to an unconscious person. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention. Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Wash contaminated clothing thoroughly with water before removing or wear gloves. Continue to rinse for at least 10 minutes. Get medical attention. Wash clothing before reuse. Clean shoes thoroughly before reuse. Move exposed person to fresh air. If it is suspected that fumes are still present, the rescuer should wear an appropriate mask or self-contained breathing apparatus. Keep person warm and at rest. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Get medical attention. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. In case of inhalation of decomposition products in a fire, symptoms may be delayed. The exposed person may need to be kept under medical surveillance for 48 hours. First aid measures Eye contact Skin contact Inhalation Ingestion : : : : 4 . Protection of first-aiders : No action shall be taken involving any personal risk or without suitable training. If it is suspected that fumes are still present, the rescuer should wear an appropriate mask or self-contained breathing apparatus. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Wash contaminated clothing thoroughly with water before removing or wear gloves. See section 11 for more detailed information on health effects and symptoms. Use an extinguishing agent suitable for the surrounding fire. Fire-fighting measures Extinguishing media Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training. In a fire or if heated, a pressure increase will occur and the container may burst. Special protective equipment for fire-fighters Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode. : 5 . Special exposure hazards : None known. Suitable : Not suitable : Hazardous combustion products : Decomposition products may include the following materials: carbon oxides nitrogen oxides sulfur oxides metal oxide/oxides Flammability of the product : 3/8 2/1/2008. 2X SDS Sample Buffer No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Avoid breathing vapor or mist. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see section 8). Stop leak if without risk. Move containers from spill area. Approach release from upwind. Prevent entry into sewers, water courses, basements or confined areas. Wash spillages into an effluent treatment plant or proceed as follows. Contain and collect spillage with non-combustible, absorbent material e.g. sand, earth, vermiculite or diatomaceous earth and place in container for disposal according to local regulations (see section 13). Dispose of via a licensed waste disposal contractor. Contaminated absorbent material may pose the same hazard as the spilled product. Note: see section 1 for emergency contact information and section 13 for waste disposal. Environmental precautions Accidental release measures : Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Personal precautions : Large spill : 6 . Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if water-soluble or absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor. Small spill : Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Put on appropriate personal protective equipment (see section 8). Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Do not get in eyes or on skin or clothing. Do not breathe vapor or mist. Do not ingest. Use only with adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container. Handling and storage Handling Storage : : 7 . GLYCEROL; GLYCERIN; GLYCERIN, ANHYDROUS; GLYCERIN, SYNTHETIC; GLYCERINE; GLYCYL ALCOHOL; 1,2,3- PROPANETRIOL; SYNTHETIC GLYCERIN; 90 TECHNICAL GLYCERIN; TRIHYDROXYPROPANE; 1,2,3- TRIHYDROXYPROPANE; GLYGERINE MIST ACGIH (United States, 0/2000). TWA: 10 mg/m³ ACGIH TLV (United States). TWA: 10 mg/m³ 8 hour(s). Form: Total particulates OSHA PEL (United States). TWA: 15 mg/m³ 8 hour(s). Form: Total dust OSHA (United States, 0/1989). Notes: Respirable TWA: 5 mg/m³ TWA: 5 mg/m³ ACGIH TLV (United States, 0/1989). Notes: Respirable TWA: 5 mg/m³ 8 hour(s). OSHA PEL 1989 (United States, 0/1989). Notes: Respirable TWA: 5 mg/m³ 8 hour(s). Exposure controls/personal protection Product name Exposure limits United States 8 . Europe 4/8 2/1/2008. 2X SDS Sample Buffer Exposure controls/personal protection 8 . Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator. Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists, gases or dusts. Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Personal protection Eyes Skin Respiratory Consult local authorities for acceptable exposure limits. : : : Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Hands : Engineering measures : Use only with adequate ventilation. If user operations generate dust, fumes, gas, vapor or mist, use process enclosures, local exhaust ventilation or other engineering controls to keep worker exposure to airborne contaminants below any recommended or statutory limits. Hygiene measures : Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. Recommended monitoring procedures : If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Environmental exposure controls : Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. Glycerol ACGIH (United States, 0/2000). TWA: 10 mg/m³ ACGIH TLV (United States). TWA: 10 mg/m³ 8 hour(s). Form: Total particulates ACGIH TLV (United States, 0/1989). Notes: Respirable TWA: 5 mg/m³ 8 hour(s). Liquid. Partially dispersible in the following materials: cold water. Odorless. Purple. Physical and chemical properties Physical state Dispersibility properties Odor Color : : : : 9 . The product is stable. Under normal conditions of storage and use, hazardous polymerization will not occur. No specific data. Under normal conditions of storage and use, hazardous decomposition products should not be produced. No specific data. Stability and reactivity Stability Conditions to avoid Materials to avoid Hazardous decomposition products : : : : 10 . 5/8 2/1/2008. 2X SDS Sample Buffer Toxicological information United States 11 . Acute toxicity sodium dodecyl sulfate LD50 Intraperitoneal Rat 210 mg/kg - LD50 Intravenous Rat 118 mg/kg - LD50 Oral Rat 1288 mg/kg - LDLo Dermal Rabbit 10 g/kg - 1,3-propanediol, 2-amino-2- (hydroxymethyl)- LD50 Intravenous Rat 1800 mg/kg - LD50 Oral Rat 5900 mg/kg - LD50 Oral Rat 5900 mg/kg - LDLo Oral Rabbit 1000 mg/kg - Glycerol LD50 Oral Rat 12600 mg/kg - LD50 Oral Guinea pig 7750 mg/kg - LD50 Oral Mouse 4090 mg/kg - Product/ingredient name Result Species Dose Exposure Conclusion/Summary : Not available. Chronic toxicity Conclusion/Summary : Not available. Carcinogenicity Conclusion/Summary : Not available. Mutagenicity Conclusion/Summary : Not available. Teratogenicity Conclusion/Summary : Not available. Reproductive toxicity Conclusion/Summary : Not available. Classification sodium dodecyl sulfate A2 - - None. - None. Glycerol - - - None. - - Product/ingredient name ACGIH EPA NIOSH NTP IARC OSHA No known significant effects or critical hazards. Chronic effects : No known significant effects or critical hazards. Carcinogenicity : No known significant effects or critical hazards. Mutagenicity No known significant effects or critical hazards. Teratogenicity Developmental effects : No known significant effects or critical hazards. Fertility effects : No known significant effects or critical hazards. : : Europe Ecological information United States Environmental effects : No known significant effects or critical hazards. 12 . Aquatic ecotoxicity Conclusion/Summary : Not available. Other adverse effects : No known significant effects or critical hazards. 6/8 2/1/2008. 2X SDS Sample Buffer The generation of waste should be avoided or minimized wherever possible. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Waste disposal Disposal considerations Disposal should be in accordance with applicable regional, national and local laws and regulations. : 13 . Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees. Hazardous waste : Within the present knowledge of the supplier, this product is not regarded as hazardous waste, as defined by EU Directive 91/689/EEC. Transport information Not available. DOT Classification Not available. - Not available. Not available. IATA-DGR Class Not available. Regulatory information UN number Proper shipping name Classes PG* - Not available. 14 . PG* : Packing group Irritating material Target organ effects HCS Classification Regulatory information U.S. Federal regulations United States inventory (TSCA 8b): All components are listed or exempted. TSCA 8(d) H and S data reporting: Clean Water Act (CWA) 307: No products were found. Clean Water Act (CWA) 311: No products were found. Clean Air Act (CAA) 112 accidental release prevention: No products were found. Clean Air Act (CAA) 112 regulated flammable substances: No products were found. Clean Air Act (CAA) 112 regulated toxic substances: No products were found. CEPA Toxic substances: None of the components are listed. Canadian ARET: None of the components are listed. Canadian NPRI: None of the components are listed. Alberta Designated Substances: None of the components are listed. Ontario Designated Substances: None of the components are listed. Quebec Designated Substances: None of the components are listed. WHMIS (Canada) Class D-2B: Material causing other toxic effects (Toxic). SARA 302/304/311/312 extremely hazardous substances: No products were found. SARA 302/304 emergency planning and notification: No products were found. SARA 302/304/311/312 hazardous chemicals: ; sodium dodecyl sulfate SARA 311/312 MSDS distribution - chemical inventory - hazard identification: : Immediate (acute) health hazard, Delayed (chronic) health hazard; sodium dodecyl sulfate: Immediate (acute) health hazard, Delayed (chronic) health hazard : : : Canada United States 15 . Canadian lists : United States inventory (TSCA 8b) : United States inventory (TSCA 8b): All components are listed or exempted. Canada inventory : Canada inventory: All components are listed or exempted. 7/8 2/1/2008. 2X SDS Sample Buffer Regulatory information 15 . Australia inventory (AICS): All components are listed or exempted. China inventory (IECSC): Not determined. Korea inventory (KECI): All components are listed or exempted. Philippines inventory (PICCS): All components are listed or exempted. Japan inventory (ENCS): All components are listed or exempted. International regulations International lists : EU regulations This product is not classified according to EU legislation. Risk phrases : : Other information Hazardous Material Information System (U.S.A.) 2 0 0 0 0 2 National Fire Protection Association (U.S.A.) Health Special Instability Flammability Health Flammability Physical hazards CAUSES RESPIRATORY TRACT, EYE AND SKIN IRRITATION. MAY BE HARMFUL IF ABSORBED THROUGH SKIN OR IF SWALLOWED. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. Label requirements : Notice to reader To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist. Date of printing Date of issue Date of previous issue Version 2/1/2008. 2/1/2008. 1 No previous validation. : : : : : : 16 . The customer is responsible for determining the PPE code for this material. Indicates information that has changed from previously issued version. Full text of R-phrases referred to in sections 2 and 3 - Europe : R11- Highly flammable. R20/21/22- Harmful by inhalation, in contact with skin and if swallowed. R21/22- Harmful in contact with skin and if swallowed. R36/37/38- Irritating to eyes, respiratory system and skin. Full text of classifications referred to in sections 2 and 3 - Europe : F - Highly flammable Xn - Harmful Xi - Irritant 8/8 2/1/2008. Lysis/Binding/Wash Buffer 4046 89856C 2/1/2008. 2/1/2008. Material Safety Data Sheet Product name Manufacturer Product No. MSDS # Validation date Print date In case of emergency Product and company identification Responsible name MSDS (Regulatory Affairs) Lysis/Binding/Wash Buffer : : : : : : : 1 . CHEMTREC: 800.424.9300 OUTSIDE US: 202.483.7616 Supplier : : Use of Substance/Preparation Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. : ThermoFisher Scientific (Milwaukee) LLC 2202 N. Bartlett Ave. Milwaukee, WI 53202 USA 414.227.3600 or 877.886.7629 ThermoFisher Scientific (Milwaukee) LLC 2202 N. Bartlett Ave. Milwaukee, WI 53202 USA 414.227.3600 or 877.886.7629 Emergency overview Hazards identification Potential acute health effects Irritating to respiratory system. Irritating to eyes. No known significant effects or critical hazards. Irritating to skin. Eyes Skin Inhalation Ingestion Physical state Liquid. WARNING ! CAUSES RESPIRATORY TRACT, EYE AND SKIN IRRITATION. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. Irritating to eyes, respiratory system and skin. Avoid exposure - obtain special instructions before use. Do not breathe vapor or mist. Avoid contact with eyes, skin and clothing. Contains material that can cause target organ damage. Use only with adequate ventilation. Keep container tightly closed and sealed until ready for use. Wash thoroughly after handling. : : : : : : Odor : Unpleasant. OSHA/HCS status : This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). 2 . Potential chronic health effects Carcinogenicity : No known significant effects or critical hazards. Mutagenicity : No known significant effects or critical hazards. Teratogenicity : No known significant effects or critical hazards. Developmental effects : No known significant effects or critical hazards. Chronic effects : Contains material that can cause target organ damage. Fertility effects : No known significant effects or critical hazards. 1/8 2/1/2008. Lysis/Binding/Wash Buffer Hazards identification 2 . See toxicological information (section 11) Medical conditions aggravated by over- exposure Pre-existing disorders involving any target organs mentioned in this MSDS as being at risk may be aggravated by over-exposure to this product. : Over-exposure signs/symptoms Ingestion : No specific data. Skin : Adverse symptoms may include the following: irritation redness Eyes : Adverse symptoms may include the following: pain or irritation watering redness Inhalation : Adverse symptoms may include the following: respiratory tract irritation coughing Target organs : Contains material which causes damage to the following organs: kidneys, mucous membranes, gastrointestinal tract, upper respiratory tract, skin, eye, lens or cornea. The preparation is not classified as dangerous according to Directive 1999/45/EC and its amendments. Classification Not classified. : GLYCEROL; GLYCERIN; GLYCERIN, ANHYDROUS; GLYCERIN, SYNTHETIC; GLYCERINE; GLYCYL ALCOHOL; 1,2,3-PROPANETRIOL; SYNTHETIC GLYCERIN; 90 TECHNICAL GLYCERIN; TRIHYDROXYPROPANE; 1,2,3- TRIHYDROXYPROPANE; GLYGERINE MIST 56-81-5 3 - 5 Polyethyleneglycol-p-isooctylphenyl Ether 9036-19-5 0.1 - 1 Composition/information on ingredients Name CAS number % United States 3 . There are no ingredients or additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section. Substance/preparation Preparation : Ingredient name CAS number % Classification EC number Glycerol 56-81-5 3 - 5 200-289-5 Xi; R36/37/38 [1] [2] Polyethyleneglycol-p-isooctylphenyl Ether 9036-19-5 0.1 - 1 Xi; R36/37/38 [1] Europe [1] Substance classified with a health or environmental hazard [2] Substance with a workplace exposure limit Occupational exposure limits, if available, are listed in section 8. 2/8 2/1/2008. Lysis/Binding/Wash Buffer Wash out mouth with water. Remove dentures if any. Move exposed person to fresh air. Keep person warm and at rest. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention. Never give anything by mouth to an unconscious person. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention. Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Continue to rinse for at least 10 minutes. Get medical attention. Wash clothing before reuse. Clean shoes thoroughly before reuse. Move exposed person to fresh air. If it is suspected that fumes are still present, the rescuer should wear an appropriate mask or self-contained breathing apparatus. Keep person warm and at rest. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Get medical attention. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. First aid measures Eye contact Skin contact Inhalation Ingestion : : : : 4 . Protection of first-aiders : No action shall be taken involving any personal risk or without suitable training. If it is suspected that fumes are still present, the rescuer should wear an appropriate mask or self-contained breathing apparatus. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. See section 11 for more detailed information on health effects and symptoms. Use an extinguishing agent suitable for the surrounding fire. Fire-fighting measures Extinguishing media Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training. In a fire or if heated, a pressure increase will occur and the container may burst. Special protective equipment for fire-fighters Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode. : 5 . Special exposure hazards : None known. Suitable : Not suitable : Hazardous combustion products : Decomposition products may include the following materials: carbon oxides Flammability of the product : No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Avoid breathing vapor or mist. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see section 8). Environmental precautions Accidental release measures : Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Personal precautions : 6 . 3/8 2/1/2008. Lysis/Binding/Wash Buffer Accidental release measures 6 . Stop leak if without risk. Move containers from spill area. Approach release from upwind. Prevent entry into sewers, water courses, basements or confined areas. Wash spillages into an effluent treatment plant or proceed as follows. Contain and collect spillage with non-combustible, absorbent material e.g. sand, earth, vermiculite or diatomaceous earth and place in container for disposal according to local regulations (see section 13). Dispose of via a licensed waste disposal contractor. Contaminated absorbent material may pose the same hazard as the spilled product. Note: see section 1 for emergency contact information and section 13 for waste disposal. Large spill : Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if water-soluble or absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor. Small spill : Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Put on appropriate personal protective equipment (see section 8). Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Do not get in eyes or on skin or clothing. Do not breathe vapor or mist. Do not ingest. Use only with adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container. Handling and storage Handling Storage : : 7 . GLYCEROL; GLYCERIN; GLYCERIN, ANHYDROUS; GLYCERIN, SYNTHETIC; GLYCERINE; GLYCYL ALCOHOL; 1,2,3- PROPANETRIOL; SYNTHETIC GLYCERIN; 90 TECHNICAL GLYCERIN; TRIHYDROXYPROPANE; 1,2,3- TRIHYDROXYPROPANE; GLYGERINE MIST ACGIH (United States, 0/2000). TWA: 10 mg/m³ ACGIH TLV (United States). TWA: 10 mg/m³ 8 hour(s). Form: Total particulates OSHA PEL (United States). TWA: 15 mg/m³ 8 hour(s). Form: Total dust OSHA (United States, 0/1989). Notes: Respirable TWA: 5 mg/m³ TWA: 5 mg/m³ ACGIH TLV (United States, 0/1989). Notes: Respirable TWA: 5 mg/m³ 8 hour(s). OSHA PEL 1989 (United States, 0/1989). Notes: Respirable TWA: 5 mg/m³ 8 hour(s). Exposure controls/personal protection Product name Exposure limits Consult local authorities for acceptable exposure limits. United States 8 . Recommended monitoring procedures : If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Europe Glycerol ACGIH (United States, 0/2000). TWA: 10 mg/m³ ACGIH TLV (United States). TWA: 10 mg/m³ 8 hour(s). Form: Total particulates ACGIH TLV (United States, 0/1989). Notes: Respirable TWA: 5 mg/m³ 8 hour(s). 4/8 2/1/2008. Lysis/Binding/Wash Buffer Exposure controls/personal protection 8 . Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator. Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists, gases or dusts. Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Personal protection Eyes Skin Respiratory : : : Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Hands : Engineering measures : Use only with adequate ventilation. If user operations generate dust, fumes, gas, vapor or mist, use process enclosures, local exhaust ventilation or other engineering controls to keep worker exposure to airborne contaminants below any recommended or statutory limits. Hygiene measures : Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. Environmental exposure controls : Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. Liquid. Partially dispersible in the following materials: cold water. Unpleasant. Colorless. Physical and chemical properties Physical state Dispersibility properties Odor Color : : : : 9 . The product is stable. Under normal conditions of storage and use, hazardous polymerization will not occur. No specific data. Under normal conditions of storage and use, hazardous decomposition products should not be produced. No specific data. Stability and reactivity Stability Conditions to avoid Materials to avoid Hazardous decomposition products : : : : 10 . Toxicological information United States 11 . Acute toxicity Product/ingredient name Result Species Dose Exposure 5/8 2/1/2008. Lysis/Binding/Wash Buffer Toxicological information 11 . Glycerol LD50 Oral Rat 12600 mg/kg - LD50 Oral Guinea pig 7750 mg/kg - LD50 Oral Mouse 4090 mg/kg - Polyethyleneglycol-p-isooctylphenyl Ether LD50 Oral Rat 4190 mg/kg - LD50 Oral Rat 3500 mg/kg - LD50 Oral Rat 2800 mg/kg - Conclusion/Summary : Not available. Chronic toxicity Conclusion/Summary : Not available. Carcinogenicity Conclusion/Summary : Not available. Mutagenicity Conclusion/Summary : Not available. Teratogenicity Conclusion/Summary : Not available. Reproductive toxicity Conclusion/Summary : Not available. Classification Glycerol - - - None. - - Polyethyleneglycol-p-isooctylphenyl Ether - - - None. - - Product/ingredient name ACGIH EPA NIOSH NTP IARC OSHA No known significant effects or critical hazards. Chronic effects : No known significant effects or critical hazards. Carcinogenicity : No known significant effects or critical hazards. Mutagenicity No known significant effects or critical hazards. Teratogenicity Developmental effects : No known significant effects or critical hazards. Fertility effects : No known significant effects or critical hazards. : : Europe Ecological information United States Environmental effects : No known significant effects or critical hazards. 12 . Aquatic ecotoxicity Conclusion/Summary : Not available. Other adverse effects : No known significant effects or critical hazards. The generation of waste should be avoided or minimized wherever possible. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Waste disposal Disposal considerations Disposal should be in accordance with applicable regional, national and local laws and regulations. : 13 . Hazardous waste : Within the present knowledge of the supplier, this product is not regarded as hazardous waste, as defined by EU Directive 91/689/EEC. 6/8 2/1/2008. Lysis/Binding/Wash Buffer Disposal considerations 13 . Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees. Transport information Not available. DOT Classification Not available. - Not available. Not available. IATA-DGR Class Not available. Regulatory information UN number Proper shipping name Classes PG* - Not available. 14 . PG* : Packing group Irritating material Target organ effects HCS Classification Regulatory information U.S. Federal regulations TSCA 8(a) PAIR: Polyethyleneglycol-p-isooctylphenyl Ether United States inventory (TSCA 8b): All components are listed or exempted. TSCA 8(d) H and S data reporting: Clean Water Act (CWA) 307: No products were found. Clean Water Act (CWA) 311: No products were found. Clean Air Act (CAA) 112 accidental release prevention: No products were found. Clean Air Act (CAA) 112 regulated flammable substances: No products were found. Clean Air Act (CAA) 112 regulated toxic substances: No products were found. CEPA Toxic substances: None of the components are listed. Canadian ARET: None of the components are listed. Canadian NPRI: The following components are listed: Octylphenol and its ethoxylates Alberta Designated Substances: None of the components are listed. Ontario Designated Substances: None of the components are listed. Quebec Designated Substances: None of the components are listed. WHMIS (Canada) Class D-2B: Material causing other toxic effects (Toxic). International regulations SARA 302/304/311/312 extremely hazardous substances: No products were found. SARA 302/304 emergency planning and notification: No products were found. SARA 302/304/311/312 hazardous chemicals: Polyethyleneglycol-p-isooctylphenyl Ether; SARA 311/312 MSDS distribution - chemical inventory - hazard identification: Polyethyleneglycol-p-isooctylphenyl Ether: Delayed (chronic) health hazard; : Immediate (acute) health hazard, Delayed (chronic) health hazard : : : Canada United States EU regulations 15 . Canadian lists : United States inventory (TSCA 8b) : United States inventory (TSCA 8b): All components are listed or exempted. Canada inventory : Canada inventory: Not determined. This product is not classified according to EU legislation. Risk phrases : : 7/8 2/1/2008. Lysis/Binding/Wash Buffer Regulatory information 15 . Australia inventory (AICS): All components are listed or exempted. China inventory (IECSC): Not determined. Korea inventory (KECI): Not determined. Philippines inventory (PICCS): All components are listed or exempted. Japan inventory (ENCS): Not determined. International lists : Other information Hazardous Material Information System (U.S.A.) 2 0 0 0 0 2 National Fire Protection Association (U.S.A.) Health Special Instability Flammability Health Flammability Physical hazards CAUSES RESPIRATORY TRACT, EYE AND SKIN IRRITATION. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. Label requirements : Notice to reader To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist. Date of printing Date of issue Date of previous issue Version 2/1/2008. 2/1/2008. 1 No previous validation. : : : : : : 16 . The customer is responsible for determining the PPE code for this material. Indicates information that has changed from previously issued version. Full text of R-phrases referred to in sections 2 and 3 - Europe : R36/37/38- Irritating to eyes, respiratory system and skin. Full text of classifications referred to in sections 2 and 3 - Europe : Xi - Irritant 8/8 2/1/2008. GST-Pak1-PBD 4048 89856E 2/1/2008. 2/1/2008. Material Safety Data Sheet Product name Manufacturer Product No. MSDS # Validation date Print date In case of emergency Product and company identification Responsible name MSDS (Regulatory Affairs) GST-Pak1-PBD : : : : : : : 1 . CHEMTREC: 800.424.9300 OUTSIDE US: 202.483.7616 Supplier : : Use of Substance/Preparation Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. : ThermoFisher Scientific (Milwaukee) LLC 2202 N. Bartlett Ave. Milwaukee, WI 53202 USA 414.227.3600 or 877.886.7629 ThermoFisher Scientific (Milwaukee) LLC 2202 N. Bartlett Ave. Milwaukee, WI 53202 USA 414.227.3600 or 877.886.7629 Dermal contact. Eye contact. Inhalation. Ingestion. Emergency overview Hazards identification Routes of entry Potential acute health effects Irritating to respiratory system. Irritating to eyes. No known significant effects or critical hazards. Irritating to skin. Eyes Skin Inhalation Ingestion Physical state Liquid. WARNING ! CAUSES RESPIRATORY TRACT, EYE AND SKIN IRRITATION. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. Irritating to eyes, respiratory system and skin. Avoid exposure - obtain special instructions before use. Do not breathe vapor or mist. Avoid contact with eyes, skin and clothing. Contains material that can cause target organ damage. Use only with adequate ventilation. Keep container tightly closed and sealed until ready for use. Wash thoroughly after handling. : : : : : : : Odor : Odorless. OSHA/HCS status : This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). 2 . Potential chronic health effects Carcinogenicity : No known significant effects or critical hazards. Mutagenicity : No known significant effects or critical hazards. Teratogenicity : No known significant effects or critical hazards. Developmental effects : No known significant effects or critical hazards. Chronic effects : Contains material that can cause target organ damage. Fertility effects : No known significant effects or critical hazards. 1/8 2/1/2008. GST-Pak1-PBD Hazards identification 2 . See toxicological information (section 11) Medical conditions aggravated by over- exposure Pre-existing disorders involving any target organs mentioned in this MSDS as being at risk may be aggravated by over-exposure to this product. : Over-exposure signs/symptoms Ingestion : No specific data. Skin : Adverse symptoms may include the following: irritation redness Eyes : Adverse symptoms may include the following: pain or irritation watering redness Inhalation : Adverse symptoms may include the following: respiratory tract irritation coughing Target organs : Contains material which causes damage to the following organs: kidneys, mucous membranes, gastrointestinal tract, upper respiratory tract, skin, eye, lens or cornea. The preparation is not classified as dangerous according to Directive 1999/45/EC and its amendments. Classification Not classified. : GLYCEROL; GLYCERIN; GLYCERIN, ANHYDROUS; GLYCERIN, SYNTHETIC; GLYCERINE; GLYCYL ALCOHOL; 1,2,3-PROPANETRIOL; SYNTHETIC GLYCERIN; 90 TECHNICAL GLYCERIN; TRIHYDROXYPROPANE; 1,2,3- TRIHYDROXYPROPANE; GLYGERINE MIST 56-81-5 10 - 20 Composition/information on ingredients Name CAS number % United States 3 . There are no ingredients or additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section. Substance/preparation Preparation : Ingredient name CAS number % Classification EC number Glycerol 56-81-5 10 - 20 200-289-5 Xi; R36/37/38 [1] [2] Europe [1] Substance classified with a health or environmental hazard [2] Substance with a workplace exposure limit Occupational exposure limits, if available, are listed in section 8. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention. Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Continue to rinse for at least 10 minutes. Get medical attention. Wash clothing before reuse. Clean shoes thoroughly before reuse. First aid measures Eye contact Skin contact : : 4 . 2/8 2/1/2008. GST-Pak1-PBD First aid measures 4 . Wash out mouth with water. Remove dentures if any. Move exposed person to fresh air. Keep person warm and at rest. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention. Never give anything by mouth to an unconscious person. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. Move exposed person to fresh air. If it is suspected that fumes are still present, the rescuer should wear an appropriate mask or self-contained breathing apparatus. Keep person warm and at rest. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Get medical attention. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. Inhalation Ingestion : : Protection of first-aiders : No action shall be taken involving any personal risk or without suitable training. If it is suspected that fumes are still present, the rescuer should wear an appropriate mask or self-contained breathing apparatus. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. See section 11 for more detailed information on health effects and symptoms. Use an extinguishing agent suitable for the surrounding fire. Fire-fighting measures Extinguishing media Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training. In a fire or if heated, a pressure increase will occur and the container may burst. Special protective equipment for fire-fighters Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode. : 5 . Special exposure hazards : None known. Suitable : Not suitable : Hazardous combustion products : Decomposition products may include the following materials: carbon oxides Flammability of the product : No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Avoid breathing vapor or mist. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see section 8). Stop leak if without risk. Move containers from spill area. Approach release from upwind. Prevent entry into sewers, water courses, basements or confined areas. Wash spillages into an effluent treatment plant or proceed as follows. Contain and collect spillage with non-combustible, absorbent material e.g. sand, earth, vermiculite or diatomaceous earth and place in container for disposal according to local regulations (see section 13). Dispose of via a licensed waste disposal contractor. Contaminated absorbent material may pose the same hazard as the spilled product. Note: see section 1 for emergency contact information and section 13 for waste disposal. Environmental precautions Accidental release measures : Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Personal precautions : Large spill : 6 . 3/8 2/1/2008. GST-Pak1-PBD Accidental release measures 6 . Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if water-soluble or absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor. Small spill : Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Put on appropriate personal protective equipment (see section 8). Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Do not get in eyes or on skin or clothing. Do not breathe vapor or mist. Do not ingest. Use only with adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container. Handling and storage Handling Storage : : 7 . GLYCEROL; GLYCERIN; GLYCERIN, ANHYDROUS; GLYCERIN, SYNTHETIC; GLYCERINE; GLYCYL ALCOHOL; 1,2,3- PROPANETRIOL; SYNTHETIC GLYCERIN; 90 TECHNICAL GLYCERIN; TRIHYDROXYPROPANE; 1,2,3- TRIHYDROXYPROPANE; GLYGERINE MIST ACGIH (United States, 0/2000). TWA: 10 mg/m³ ACGIH TLV (United States). TWA: 10 mg/m³ 8 hour(s). Form: Total particulates OSHA PEL (United States). TWA: 15 mg/m³ 8 hour(s). Form: Total dust OSHA (United States, 0/1989). Notes: Respirable TWA: 5 mg/m³ TWA: 5 mg/m³ ACGIH TLV (United States, 0/1989). Notes: Respirable TWA: 5 mg/m³ 8 hour(s). OSHA PEL 1989 (United States, 0/1989). Notes: Respirable TWA: 5 mg/m³ 8 hour(s). Exposure controls/personal protection Product name Exposure limits Consult local authorities for acceptable exposure limits. United States 8 . Engineering measures : Use only with adequate ventilation. If user operations generate dust, fumes, gas, vapor or mist, use process enclosures, local exhaust ventilation or other engineering controls to keep worker exposure to airborne contaminants below any recommended or statutory limits. Recommended monitoring procedures : If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Europe Glycerol ACGIH (United States, 0/2000). TWA: 10 mg/m³ ACGIH TLV (United States). TWA: 10 mg/m³ 8 hour(s). Form: Total particulates ACGIH TLV (United States, 0/1989). Notes: Respirable TWA: 5 mg/m³ 8 hour(s). 4/8 2/1/2008. GST-Pak1-PBD Exposure controls/personal protection 8 . Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator. Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists, gases or dusts. Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Personal protection Eyes Skin Respiratory : : : Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Hands : Hygiene measures : Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. Environmental exposure controls : Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. Liquid. 7.2 Partially dispersible in the following materials: cold water. Soluble in the following materials: cold water. Odorless. Colorless. Physical and chemical properties Physical state pH Dispersibility properties Solubility Odor Color : : : : : : 9 . The product is stable. Under normal conditions of storage and use, hazardous polymerization will not occur. No specific data. Under normal conditions of storage and use, hazardous decomposition products should not be produced. No specific data. Stability and reactivity Stability Conditions to avoid Materials to avoid Hazardous decomposition products : : : : 10 . Toxicological information United States 11 . Acute toxicity Glycerol LD50 Oral Rat 12600 mg/kg - LD50 Oral Guinea pig 7750 mg/kg - LD50 Oral Mouse 4090 mg/kg - Product/ingredient name Result Species Dose Exposure Conclusion/Summary : Not available. Chronic toxicity Conclusion/Summary : Not available. 5/8 2/1/2008. GST-Pak1-PBD Toxicological information 11 . Carcinogenicity Conclusion/Summary : Not available. Mutagenicity Conclusion/Summary : Not available. Teratogenicity Conclusion/Summary : Not available. Reproductive toxicity Conclusion/Summary : Not available. Classification Glycerol - - - None. - - Product/ingredient name ACGIH EPA NIOSH NTP IARC OSHA No known significant effects or critical hazards. Chronic effects : No known significant effects or critical hazards. Carcinogenicity : No known significant effects or critical hazards. Mutagenicity No known significant effects or critical hazards. Teratogenicity Developmental effects : No known significant effects or critical hazards. Fertility effects : No known significant effects or critical hazards. : : Europe Ecological information United States Environmental effects : No known significant effects or critical hazards. 12 . Aquatic ecotoxicity Conclusion/Summary : Not available. Other adverse effects : No known significant effects or critical hazards. The generation of waste should be avoided or minimized wherever possible. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Waste disposal Disposal considerations Disposal should be in accordance with applicable regional, national and local laws and regulations. : 13 . Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees. Hazardous waste : Within the present knowledge of the supplier, this product is not regarded as hazardous waste, as defined by EU Directive 91/689/EEC. Transport information Not available. DOT Classification Not available. - Not available. Not available. IATA-DGR Class Not available. Regulatory information UN number Proper shipping name Classes PG* - Not available. 14 . 6/8 2/1/2008. GST-Pak1-PBD Transport information 14 . PG* : Packing group Irritating material Target organ effects HCS Classification Regulatory information U.S. Federal regulations United States inventory (TSCA 8b): Not determined. TSCA 8(d) H and S data reporting: Clean Water Act (CWA) 307: No products were found. Clean Water Act (CWA) 311: No products were found. Clean Air Act (CAA) 112 accidental release prevention: No products were found. Clean Air Act (CAA) 112 regulated flammable substances: No products were found. Clean Air Act (CAA) 112 regulated toxic substances: No products were found. CEPA Toxic substances: None of the components are listed. Canadian ARET: None of the components are listed. Canadian NPRI: None of the components are listed. Alberta Designated Substances: None of the components are listed. Ontario Designated Substances: None of the components are listed. Quebec Designated Substances: None of the components are listed. Australia inventory (AICS): Not determined. China inventory (IECSC): Not determined. Korea inventory (KECI): Not determined. Philippines inventory (PICCS): Not determined. Japan inventory (ENCS): Not determined. WHMIS (Canada) Not controlled under WHMIS (Canada). International regulations International lists SARA 302/304/311/312 extremely hazardous substances: No products were found. SARA 302/304 emergency planning and notification: No products were found. SARA 302/304/311/312 hazardous chemicals: SARA 311/312 MSDS distribution - chemical inventory - hazard identification: : Immediate (acute) health hazard, Delayed (chronic) health hazard : : : : Canada United States EU regulations 15 . Canadian lists : United States inventory (TSCA 8b) : United States inventory (TSCA 8b): Not determined. Canada inventory : Canada inventory: Not determined. This product is not classified according to EU legislation. Risk phrases : : Other information Hazardous Material Information System (U.S.A.) 2 0 0 Health Flammability Physical hazards CAUSES RESPIRATORY TRACT, EYE AND SKIN IRRITATION. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. Label requirements : : 16 . 7/8 2/1/2008. GST-Pak1-PBD Other information 16 . 0 0 2 National Fire Protection Association (U.S.A.) Health Special Instability Flammability Notice to reader To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist. Date of printing Date of issue Date of previous issue Version 2/1/2008. 2/1/2008. 1 No previous validation. : : : : : The customer is responsible for determining the PPE code for this material. Indicates information that has changed from previously issued version. Full text of R-phrases referred to in sections 2 and 3 - Europe : R36/37/38- Irritating to eyes, respiratory system and skin. Full text of classifications referred to in sections 2 and 3 - Europe : Xi - Irritant 8/8 2/1/2008. Anti-Rac1 Antibody 4049 89856D 2/1/2008. 2/1/2008. Material Safety Data Sheet Product name Manufacturer Product No. MSDS # Validation date Print date In case of emergency Product and company identification Responsible name MSDS (Regulatory Affairs) Anti-Rac1 Antibody : : : : : : : 1 . CHEMTREC: 800.424.9300 OUTSIDE US: 202.483.7616 Supplier : : Use of Substance/Preparation Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. : ThermoFisher Scientific (Milwaukee) LLC 2202 N. Bartlett Ave. Milwaukee, WI 53202 USA 414.227.3600 or 877.886.7629 ThermoFisher Scientific (Milwaukee) LLC 2202 N. Bartlett Ave. Milwaukee, WI 53202 USA 414.227.3600 or 877.886.7629 Emergency overview Hazards identification Potential acute health effects No known significant effects or critical hazards. No known significant effects or critical hazards. No known significant effects or critical hazards. No known significant effects or critical hazards. Eyes Skin Inhalation Ingestion Physical state Liquid. NOT EXPECTED TO PRODUCE SIGNIFICANT ADVERSE HEALTH EFFECTS WHEN THE RECOMMENDED INSTRUCTIONS FOR USE ARE FOLLOWED. No known significant effects or critical hazards. Avoid prolonged contact with eyes, skin and clothing. : : : : : : OSHA/HCS status : While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product. 2 . Potential chronic health effects Carcinogenicity : No known significant effects or critical hazards. Mutagenicity : No known significant effects or critical hazards. Teratogenicity : No known significant effects or critical hazards. Developmental effects : No known significant effects or critical hazards. Chronic effects : No known significant effects or critical hazards. Fertility effects : No known significant effects or critical hazards. Over-exposure signs/symptoms Ingestion : No specific data. Skin : No specific data. Inhalation : No specific data. 1/6 2/1/2008. Anti-Rac1 Antibody Hazards identification 2 . See toxicological information (section 11) Medical conditions aggravated by over- exposure None known. : Eyes : No specific data. The substance is not classified as dangerous according to Directive 67/548/EEC and its amendments. Classification Not classified. : Composition/information on ingredients 3 . There are no ingredients or additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section. Substance/preparation Substance : Wash out mouth with water. Remove dentures if any. Move exposed person to fresh air. Keep person warm and at rest. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention if symptoms occur. Never give anything by mouth to an unconscious person. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs. Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur. Wash clothing before reuse. Clean shoes thoroughly before reuse. Move exposed person to fresh air. Keep person warm and at rest. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. Get medical attention if symptoms occur. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. First aid measures Eye contact Skin contact Inhalation Ingestion : : : : 4 . Protection of first-aiders : No action shall be taken involving any personal risk or without suitable training. See section 11 for more detailed information on health effects and symptoms. Use an extinguishing agent suitable for the surrounding fire. Fire-fighting measures Extinguishing media Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training. In a fire or if heated, a pressure increase will occur and the container may burst. Special protective equipment for fire-fighters Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode. : 5 . Special exposure hazards : None known. Suitable : Not suitable : Hazardous combustion products : No specific data. Flammability of the product : 2/6 2/1/2008. Anti-Rac1 Antibody No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Avoid breathing vapor or mist. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see section 8). Stop leak if without risk. Move containers from spill area. Approach release from upwind. Prevent entry into sewers, water courses, basements or confined areas. Wash spillages into an effluent treatment plant or proceed as follows. Contain and collect spillage with non-combustible, absorbent material e.g. sand, earth, vermiculite or diatomaceous earth and place in container for disposal according to local regulations (see section 13). Dispose of via a licensed waste disposal contractor. Contaminated absorbent material may pose the same hazard as the spilled product. Note: see section 1 for emergency contact information and section 13 for waste disposal. Environmental precautions Accidental release measures : Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Personal precautions : Large spill : 6 . Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if water-soluble or absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor. Small spill : Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Put on appropriate personal protective equipment (see section 8). Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Do not ingest. Avoid contact with eyes, skin and clothing. Avoid breathing vapor or mist. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container. Handling and storage Handling Storage : : 7 . Exposure controls/personal protection Personal protection Consult local authorities for acceptable exposure limits. 8 . Engineering measures : No special ventilation requirements. Good general ventilation should be sufficient to control worker exposure to airborne contaminants. If this product contains ingredients with exposure limits, use process enclosures, local exhaust ventilation or other engineering controls to keep worker exposure below any recommended or statutory limits. Hygiene measures : Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. Recommended monitoring procedures : If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Europe No exposure limit value known. 3/6 2/1/2008. Anti-Rac1 Antibody Exposure controls/personal protection 8 . Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator. Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists, gases or dusts. Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Eyes Skin Respiratory : : : Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Hands : Environmental exposure controls : Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. Liquid. Physical and chemical properties Physical state : 9 . The product is stable. Under normal conditions of storage and use, hazardous polymerization will not occur. No specific data. Under normal conditions of storage and use, hazardous decomposition products should not be produced. No specific data. Stability and reactivity Stability Conditions to avoid Materials to avoid Hazardous decomposition products : : : : 10 . Toxicological information United States 11 . Acute toxicity Conclusion/Summary : Not available. Chronic toxicity Conclusion/Summary : Not available. Carcinogenicity Conclusion/Summary : Not available. Mutagenicity Conclusion/Summary : Not available. Teratogenicity Conclusion/Summary : Not available. Reproductive toxicity Conclusion/Summary : Not available. No known significant effects or critical hazards. Chronic effects : No known significant effects or critical hazards. Carcinogenicity : No known significant effects or critical hazards. Mutagenicity No known significant effects or critical hazards. Teratogenicity : : Europe 4/6 2/1/2008. Anti-Rac1 Antibody Toxicological information 11 . Developmental effects : No known significant effects or critical hazards. Fertility effects : No known significant effects or critical hazards. Ecological information United States Environmental effects : No known significant effects or critical hazards. 12 . Aquatic ecotoxicity Conclusion/Summary : Not available. Other adverse effects : No known significant effects or critical hazards. The generation of waste should be avoided or minimized wherever possible. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Waste disposal Disposal considerations Disposal should be in accordance with applicable regional, national and local laws and regulations. : 13 . Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees. Hazardous waste : Within the present knowledge of the supplier, this product is not regarded as hazardous waste, as defined by EU Directive 91/689/EEC. Transport information Not available. DOT Classification Not available. - Not available. Not available. IATA-DGR Class Not available. Regulatory information UN number Proper shipping name Classes PG* - Not available. 14 . PG* : Packing group Not regulated. HCS Classification Regulatory information U.S. Federal regulations United States inventory (TSCA 8b): Not determined. Clean Water Act (CWA) 307: No products were found. Clean Water Act (CWA) 311: No products were found. Clean Air Act (CAA) 112 accidental release prevention: No products were found. Clean Air Act (CAA) 112 regulated flammable substances: No products were found. Clean Air Act (CAA) 112 regulated toxic substances: No products were found. SARA 302/304/311/312 extremely hazardous substances: No products were found. SARA 302/304 emergency planning and notification: No products were found. SARA 302/304/311/312 hazardous chemicals: No products were found. SARA 311/312 MSDS distribution - chemical inventory - hazard identification: No products were found. : : United States 15 . United States inventory (TSCA 8b) : United States inventory (TSCA 8b): Not determined. 5/6 2/1/2008. Anti-Rac1 Antibody Regulatory information 15 . CEPA Toxic substances: None of the components are listed. Canadian ARET: None of the components are listed. Canadian NPRI: None of the components are listed. Alberta Designated Substances: None of the components are listed. Ontario Designated Substances: None of the components are listed. Quebec Designated Substances: None of the components are listed. Australia inventory (AICS): Not determined. China inventory (IECSC): Not determined. Korea inventory (KECI): Not determined. Philippines inventory (PICCS): Not determined. Japan inventory (ENCS): Not determined. WHMIS (Canada) Not controlled under WHMIS (Canada). International regulations International lists : : Canada EU regulations Canadian lists : Canada inventory : Canada inventory: Not determined. This product is not classified according to EU legislation. Risk phrases : : Other information Hazardous Material Information System (U.S.A.) 0 0 0 0 0 0 National Fire Protection Association (U.S.A.) Health Special Instability Flammability Health Flammability Physical hazards NOT EXPECTED TO PRODUCE SIGNIFICANT ADVERSE HEALTH EFFECTS WHEN THE RECOMMENDED INSTRUCTIONS FOR USE ARE FOLLOWED. Label requirements : Notice to reader To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist. Date of printing Date of issue Date of previous issue Version 2/1/2008. 2/1/2008. 1 No previous validation. : : : : : : 16 . The customer is responsible for determining the PPE code for this material. Indicates information that has changed from previously issued version. 6/6 2/1/2008. GDP (100 mM) 4050 89856F 2/1/2008. 2/1/2008. Material Safety Data Sheet Product name Manufacturer Product No. MSDS # Validation date Print date In case of emergency Product and company identification Responsible name MSDS (Regulatory Affairs) GDP (100 mM) : : : : : : : 1 . CHEMTREC: 800.424.9300 OUTSIDE US: 202.483.7616 Supplier : : Use of Substance/Preparation Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. : ThermoFisher Scientific (Milwaukee) LLC 2202 N. Bartlett Ave. Milwaukee, WI 53202 USA 414.227.3600 or 877.886.7629 ThermoFisher Scientific (Milwaukee) LLC 2202 N. Bartlett Ave. Milwaukee, WI 53202 USA 414.227.3600 or 877.886.7629 Emergency overview Hazards identification Potential acute health effects No known significant effects or critical hazards. No known significant effects or critical hazards. No known significant effects or critical hazards. No known significant effects or critical hazards. Eyes Skin Inhalation Ingestion Physical state Liquid. NOT EXPECTED TO PRODUCE SIGNIFICANT ADVERSE HEALTH EFFECTS WHEN THE RECOMMENDED INSTRUCTIONS FOR USE ARE FOLLOWED. No known significant effects or critical hazards. Avoid prolonged contact with eyes, skin and clothing. : : : : : : Odor : Odorless. OSHA/HCS status : While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product. 2 . Potential chronic health effects Carcinogenicity : No known significant effects or critical hazards. Mutagenicity : No known significant effects or critical hazards. Teratogenicity : No known significant effects or critical hazards. Developmental effects : No known significant effects or critical hazards. Chronic effects : No known significant effects or critical hazards. Fertility effects : No known significant effects or critical hazards. Over-exposure signs/symptoms Ingestion : No specific data. Inhalation : No specific data. 1/7 2/1/2008. GDP (100 mM) Hazards identification 2 . See toxicological information (section 11) Medical conditions aggravated by over- exposure None known. : Skin : No specific data. Eyes : No specific data. The preparation is not classified as dangerous according to Directive 1999/45/EC and its amendments. Classification Not classified. : Composition/information on ingredients 3 . There are no ingredients or additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section. Substance/preparation Preparation : Wash out mouth with water. Remove dentures if any. Move exposed person to fresh air. Keep person warm and at rest. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention if symptoms occur. Never give anything by mouth to an unconscious person. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs. Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur. Wash clothing before reuse. Clean shoes thoroughly before reuse. Move exposed person to fresh air. Keep person warm and at rest. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. Get medical attention if symptoms occur. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. First aid measures Eye contact Skin contact Inhalation Ingestion : : : : 4 . Protection of first-aiders : No action shall be taken involving any personal risk or without suitable training. See section 11 for more detailed information on health effects and symptoms. Use an extinguishing agent suitable for the surrounding fire. Fire-fighting measures Extinguishing media Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training. In a fire or if heated, a pressure increase will occur and the container may burst. Special protective equipment for fire-fighters Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode. : 5 . Special exposure hazards : None known. Suitable : Not suitable : Hazardous combustion products : No specific data. Flammability of the product : 2/7 2/1/2008.