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Abstracts / Journal of Biotec
1-Y-043
thanol production from soybean sirup by co-cultivation of
spergillus oryzae and Saccharomyces cerevisiae
iangyang Ge 1,∗, He Qian 2, Weiguo Zhang 1
The Key Laboratory of Industrial Biotechnology, Ministry of Educa-
ion, School of Biotechnology, JiangNan University, 1800# Lihu Road,
uxi-214122, JiangSu Province, PR China
The State Key Laboratory of Food Science&Technology, School of Food
cience, JiangNan University, PR China
-mail address: xyge168@126.com (X. Ge).
oybean sirup was fermented to ethanol using mixed culture
f Aspergillus oryzae and Saccharomyces cerevisiae. The syntheses
f alpha-galactosidase and invertase from A. oryzae L-09 were
nhanced significantly by the inoculation of S. cerevisiae Z-06 at
he beginning of the culture (Abu et al., 2005). To balance the
rowth of the both strains and enhance the enzymes produc-
ion, the aeration rate of the culture was controlled at 3.0 l/min
n the beginning 24 h, and at 5.0 l/min in the following 30 h
f the culture; then S. cerevisiae cell concentration of 108 ml−1,
nzyme activities of 45.5 and 96.7 IU ml−1 for alpha-galactosidase
nd invertase (8.2- and 4.1-fold greater than that for the single
train, respectively) were obtained simultaneously. The informa-
ion obtained by the mixture culture signified that the inoculation
ime and aeration rate influenced the growth performance and
nzymes production of the both strains significantly (Alfenore
t al., 2004; Shankar and Mulimani, 2007). Then additional soy-
ean sirup was direct convert to ethanol at the concentration
f 158.4 g l−1 in 54 h in the following fed-batch culture, and the
onversion efficiency of total sugar to ethanol was 96.6% of the
heoretical yield. This present type of fermentation process might
ake soybean sirup a promising raw material for bioethanol
roduction.
eferences
bu, E.A., Ado, S.A., James, D.B., 2005.