Elution Buffer for 6xHis Kit
2672
0078130 1900322
5/20/2008.
5/20/2008.
Material Safety Data Sheet
Product name
Manufacturer
Product No.
MSDS #
Validation date
Print date
In case of emergency
Product and company identification
Responsible name
MSDS (Regulatory Affairs)
Elution Buffer for 6xHis Kit
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1 .
CHEMTREC:
800.424.9300
OUTSIDE US:
202.483.7616
Supplier
: Thermo Fisher Scientific
Pierce Biotechnology
P.O. Box 117
Rockford, IL 61105
United States
815.968.0747 or
800.874.3723
:
Use of
Substance/Preparation
Refer to the instruction booklet for
proper and intended use.
Otherwise, contact supplier for
specific applications.
: Thermo Fisher Scientific
Pierce Biotechnology
P.O. Box 117
Rockford, IL 61105
United States
815.968.0747
800.874.3723
Emergency overview
Hazards identification
Potential acute health effects
Corrosive to the respiratory system. Exposure to decomposition products may cause a
health hazard. Serious effects may be delayed following exposure.
Corrosive to eyes. Causes burns.
Harmful if swallowed. May cause burns to mouth, throat and stomach.
Corrosive to the skin. Causes burns.
Eyes
Skin
Inhalation
Ingestion
Physical state
Liquid. [Clear sparkling liquid.]
DANGER !
CAUSES RESPIRATORY TRACT, EYE AND SKIN BURNS. MAY BE HARMFUL IF
SWALLOWED. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN
DAMAGE.
Corrosive to the eyes, skin and respiratory system. Causes burns. Harmful if
swallowed. Avoid exposure - obtain special instructions before use. Do not breathe
vapor or mist. Do not ingest. Do not get in eyes or on skin or clothing. Contains
material that can cause target organ damage. Use only with adequate ventilation. Keep
container tightly closed and sealed until ready for use. Wash thoroughly after handling.
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OSHA/HCS status
: This material is considered hazardous by the OSHA Hazard Communication Standard
(29 CFR 1910.1200).
2 .
Potential chronic health effects
Carcinogenicity
: No known significant effects or critical hazards.
Mutagenicity
: No known significant effects or critical hazards.
Teratogenicity
: No known significant effects or critical hazards.
Developmental effects
: No known significant effects or critical hazards.
Chronic effects
: Contains material that can cause target organ damage.
Fertility effects
: No known significant effects or critical hazards.
1/9
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Elution Buffer for 6xHis Kit
Hazards identification
2 .
See toxicological information (section 11)
Medical conditions
aggravated by over-
exposure
Pre-existing digestive disorders and disorders involving any other target organs
mentioned in this MSDS as being at risk may be aggravated by over-exposure to this
product.
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Over-exposure signs/symptoms
Ingestion
: Adverse symptoms may include the following:
stomach pains
Skin
: Adverse symptoms may include the following:
pain or irritation
redness
blistering may occur
Eyes
: Adverse symptoms may include the following:
pain
watering
redness
Inhalation
: Adverse symptoms may include the following:
respiratory tract irritation
coughing
Target organs
: Contains material which causes damage to the following organs: mucous membranes,
skin, stomach.
Contains material which may cause damage to the following organs: kidneys, upper
respiratory tract, eye, lens or cornea.
The preparation is not classified as dangerous according to Directive 1999/45/EC and its amendments.
Classification
Not classified.
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Elution Buffer Ingredient 5
7 - 10
Elution Buffer Ingredient 1
1 - 3
Imidazole
288-32-4
1 - 3
Composition/information on ingredients
Name
CAS number
%
United States
3 .
There are no ingredients or additional ingredients present which, within the current knowledge of the supplier and
in the concentrations applicable, are classified as hazardous to health or the environment and hence require
reporting in this section.
Substance/preparation
Preparation
:
Ingredient name
CAS
number
%
Classification
EC number
Elution Buffer Ingredient 5
7 - 10
Xi;
R36/37/38
[1] [2]
Imidazole
288-32-4
1 - 3
206-019-2
Xn; R22
C; R34
[1]
Europe
[1] Substance classified with a health or environmental hazard
[2] Substance with a workplace exposure limit
Occupational exposure limits, if available, are listed in section 8.
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Elution Buffer for 6xHis Kit
Get medical attention immediately. Wash out mouth with water. Remove dentures if
any. Move exposed person to fresh air. Keep person warm and at rest. If material has
been swallowed and the exposed person is conscious, give small quantities of water to
drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not
induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the
head should be kept low so that vomit does not enter the lungs. Chemical burns must
be treated promptly by a physician. Never give anything by mouth to an unconscious
person. If unconscious, place in recovery position and get medical attention
immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or
waistband.
Get medical attention immediately. Immediately flush eyes with plenty of water for at
least 15 minutes, occasionally lifting the upper and lower eyelids. Check for and remove
any contact lenses. Chemical burns must be treated promptly by a physician.
Get medical attention immediately. Flush contaminated skin with plenty of water.
Remove contaminated clothing and shoes. Wash contaminated clothing thoroughly with
water before removing or wear gloves. Continue to rinse for at least 10 minutes.
Chemical burns must be treated promptly by a physician. Wash clothing before reuse.
Clean shoes thoroughly before reuse.
Get medical attention immediately. Move exposed person to fresh air. If it is suspected
that fumes are still present, the rescuer should wear an appropriate mask or self-
contained breathing apparatus. Keep person warm and at rest. If not breathing, if
breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen
by trained personnel. It may be dangerous to the person providing aid to give mouth-to-
mouth resuscitation. If unconscious, place in recovery position and get medical attention
immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or
waistband. In case of inhalation of decomposition products in a fire, symptoms may be
delayed. The exposed person may need to be kept under medical surveillance for 48
hours.
First aid measures
Eye contact
Skin contact
Inhalation
Ingestion
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Protection of first-aiders
: No action shall be taken involving any personal risk or without suitable training. If it is
suspected that fumes are still present, the rescuer should wear an appropriate mask or
self-contained breathing apparatus. It may be dangerous to the person providing aid to
give mouth-to-mouth resuscitation. Wash contaminated clothing thoroughly with water
before removing or wear gloves.
See section 11 for more detailed information on health effects and symptoms.
Use an extinguishing agent suitable for the surrounding fire.
Fire-fighting measures
Extinguishing media
Promptly isolate the scene by removing all persons from the vicinity of the incident if
there is a fire. No action shall be taken involving any personal risk or without suitable
training.
In a fire or if heated, a pressure increase will occur and the container may burst.
Special protective
equipment for fire-fighters
Fire-fighters should wear appropriate protective equipment and self-contained breathing
apparatus (SCBA) with a full face-piece operated in positive pressure mode.
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5 .
Special exposure hazards
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None known.
Suitable
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Not suitable
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Hazardous combustion
products
: Decomposition products may include the following materials:
carbon oxides
nitrogen oxides
halogenated compounds
metal oxide/oxides
Flammability of the product
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Elution Buffer for 6xHis Kit
No action shall be taken involving any personal risk or without suitable training.
Evacuate surrounding areas. Keep unnecessary and unprotected personnel from
entering. Do not touch or walk through spilled material. Avoid breathing vapor or mist.
Provide adequate ventilation. Wear appropriate respirator when ventilation is
inadequate. Put on appropriate personal protective equipment (see section 8).
Stop leak if without risk. Move containers from spill area. Approach release from
upwind. Prevent entry into sewers, water courses, basements or confined areas. Wash
spillages into an effluent treatment plant or proceed as follows. Contain and collect
spillage with non-combustible, absorbent material e.g. sand, earth, vermiculite or
diatomaceous earth and place in container for disposal according to local regulations
(see section 13). Dispose of via a licensed waste disposal contractor. Contaminated
absorbent material may pose the same hazard as the spilled product. Note: see section
1 for emergency contact information and section 13 for waste disposal.
Environmental precautions
Accidental release measures
: Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains
and sewers. Inform the relevant authorities if the product has caused environmental
pollution (sewers, waterways, soil or air).
Personal precautions
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Large spill
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Stop leak if without risk. Move containers from spill area. Dilute with water and mop up
if water-soluble or absorb with an inert dry material and place in an appropriate waste
disposal container. Dispose of via a licensed waste disposal contractor.
Small spill
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Store in accordance with local regulations. Store in original container protected from
direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials
(see section 10) and food and drink. Keep container tightly closed and sealed until ready
for use. Containers that have been opened must be carefully resealed and kept upright
to prevent leakage. Do not store in unlabeled containers. Use appropriate containment
to avoid environmental contamination.
Put on appropriate personal protective equipment (see section 8). Eating, drinking and
smoking should be prohibited in areas where this material is handled, stored and
processed. Workers should wash hands and face before eating, drinking and smoking.
Do not get in eyes or on skin or clothing. Do not breathe vapor or mist. Do not ingest.
Use only with adequate ventilation. Wear appropriate respirator when ventilation is
inadequate. Keep in the original container or an approved alternative made from a
compatible material, kept tightly closed when not in use. Empty containers retain
product residue and can be hazardous. Do not reuse container.
Handling and storage
Handling
Storage
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7 .
Elution Buffer Ingredient 5
ACGIH (United States).
TWA: 10 mg/m³
ACGIH TLV (United States).
TWA: 10 mg/m³ 8 hour(s). Form: Total particulates
OSHA PEL (United States).
TWA: 15 mg/m³ 8 hour(s). Form: Total dust
ACGIH TLV (United States, 1/2007).
TWA: 10 mg/m³ 8 hour(s). Form: Mist
OSHA PEL (United States, 11/2006).
TWA: 5 mg/m³ 8 hour(s). Form: Respirable fraction
TWA: 15 mg/m³ 8 hour(s). Form: Total dust
OSHA PEL 1989 (United States, 3/1989).
TWA: 5 mg/m³ 8 hour(s). Form: Respirable fraction
TWA: 10 mg/m³ 8 hour(s). Form: Total dust
ACGIH TLV (United States).
TWA: 10 mg/m³ 8 hour(s). Form: Total particulates
Exposure controls/personal protection
Product name
Exposure limits
United States
8 .
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Elution Buffer for 6xHis Kit
Exposure controls/personal protection
8 .
OSHA PEL (United States).
TWA: 15 mg/m³ 8 hour(s). Form: Total dust
OSHA PEL (United States). Notes: Respirable
TWA: 15 mg/m³ 8 hour(s).
Use a properly fitted, air-purifying or air-fed respirator complying with an approved
standard if a risk assessment indicates this is necessary. Respirator selection must be
based on known or anticipated exposure levels, the hazards of the product and the safe
working limits of the selected respirator.
Safety eyewear complying with an approved standard should be used when a risk
assessment indicates this is necessary to avoid exposure to liquid splashes, mists,
gases or dusts.
Personal protective equipment for the body should be selected based on the task being
performed and the risks involved and should be approved by a specialist before handling
this product.
Personal protection
Eyes
Skin
Respiratory
Consult local authorities for acceptable exposure limits.
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Chemical-resistant, impervious gloves complying with an approved standard should be
worn at all times when handling chemical products if a risk assessment indicates this is
necessary.
Hands
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Engineering measures
: Use only with adequate ventilation. If user operations generate dust, fumes, gas, vapor
or mist, use process enclosures, local exhaust ventilation or other engineering controls
to keep worker exposure to airborne contaminants below any recommended or statutory
limits.
Hygiene measures
: Wash hands, forearms and face thoroughly after handling chemical products, before
eating, smoking and using the lavatory and at the end of the working period. Appropriate
techniques should be used to remove potentially contaminated clothing. Wash
contaminated clothing before reusing. Ensure that eyewash stations and safety showers
are close to the workstation location.
Recommended monitoring
procedures
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If this product contains ingredients with exposure limits, personal, workplace atmosphere
or biological monitoring may be required to determine the effectiveness of the ventilation
or other control measures and/or the necessity to use respiratory protective equipment.
Environmental exposure
controls
: Emissions from ventilation or work process equipment should be checked to ensure they
comply with the requirements of environmental protection legislation. In some cases,
fume scrubbers, filters or engineering modifications to the process equipment will be
necessary to reduce emissions to acceptable levels.
Europe
Elution Buffer Ingredient 5
ACGIH (United States).
TWA: 10 mg/m³
ACGIH TLV (United States).
TWA: 10 mg/m³ 8 hour(s). Form: Total particulates
TWA: 10 mg/m³ 8 hour(s). Form: Mist
TWA: 10 mg/m³ 8 hour(s). Form: Total particulates
Liquid. [Clear sparkling liquid.]
6.8
Partially dispersible in the following materials: cold water.
Colorless.
Physical and chemical properties
Physical state
pH
Dispersibility properties
Color
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9 .
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Elution Buffer for 6xHis Kit
The product is stable. Under normal conditions of storage and use, hazardous
polymerization will not occur.
No specific data.
Will not occur.
Under normal conditions of storage and use, hazardous decomposition products should
not be produced.
No specific data.
Stability and reactivity
Chemical stability
Conditions to avoid
Incompatible materials
Hazardous decomposition
products
Possibility of hazardous
reactions
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10 .
Toxicological information
United States
11 .
Acute toxicity
Elution Buffer Ingredient 5
LD50 Dermal
Rabbit
>10 g/kg
-
LD50
Intraperitoneal
Rat
4420 mg/kg
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LD50 Intravenous Rat
5566 mg/kg
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LD50 Oral
Rat
12600 mg/kg
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LD50 Oral
Rat
12600 mg/kg
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LD50 Oral
Mouse
4090 mg/kg
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LD50
Subcutaneous
Rat
100 mg/kg
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LDLo
Intramuscular
Rat
10 mg/kg
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TDLo
Intramuscular
Rat
8 mL/kg
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TDLo
Intramuscular
Rat
5000 mg/kg
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Elution Buffer Ingredient 1
LD50 Dermal
Rabbit
>10 g/kg
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LD50
Intraperitoneal
Rat
2600 mg/kg
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LD50 Oral
Rat
3000 mg/kg
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LDLo
Intraperitoneal
Rat
3.72 g/kg
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LDLo
Subcutaneous
Rat
3500 mg/kg
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1h-imidazole
LD50 Oral
Guinea pig
760 mg/kg
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LD50 Oral
Mouse
880 mg/kg
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LD50 Oral
Rat
220 mg/kg
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LD50 Oral
Rat
220 mg/kg
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LD50
Subcutaneous
Rat
626 mg/kg
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Product/ingredient name
Result
Species
Dose
Exposure
Conclusion/Summary
: Not available.
Chronic toxicity
Conclusion/Summary
: Not available.
Carcinogenicity
Conclusion/Summary
: Not available.
Mutagenicity
Classification
Elution Buffer Ingredient 5
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-
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None.
Elution Buffer Ingredient 1
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None.
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None.
1h-imidazole
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None.
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None.
Product/ingredient name
ACGIH
EPA
NIOSH
NTP
IARC
OSHA
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Elution Buffer for 6xHis Kit
Toxicological information
11 .
Conclusion/Summary
: Not available.
Teratogenicity
Conclusion/Summary
: Not available.
Reproductive toxicity
No known significant effects or critical hazards.
Chronic effects
:
No known significant effects or critical hazards.
Carcinogenicity
:
No known significant effects or critical hazards.
Mutagenicity
No known significant effects or critical hazards.
Teratogenicity
Developmental effects
: No known significant effects or critical hazards.
Fertility effects
: No known significant effects or critical hazards.
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Europe
Not available.
Conclusion/Summary
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Ecological information
United States
Environmental effects
: No known significant effects or critical hazards.
12 .
Aquatic ecotoxicity
Conclusion/Summary
: Not available.
Other adverse effects
: No known significant effects or critical hazards.
The generation of waste should be avoided or minimized wherever possible. Dispose of
surplus and non-recyclable products via a licensed waste disposal contractor. Disposal
of this product, solutions and any by-products should at all times comply with the
requirements of environmental protection and waste disposal legislation and any regional
local authority requirements. Avoid dispersal of spilled material and runoff and contact
with soil, waterways, drains and sewers.
Waste disposal
Disposal considerations
Disposal should be in accordance with applicable regional, national and local laws and regulations.
:
13 .
Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION
for additional handling information and protection of employees.
Hazardous waste
: Within the present knowledge of the supplier, this product is not regarded as
hazardous waste, as defined by EU Directive 91/689/EEC.
Transport information
-
DOT Classification
-
-
-
Not
regulated.
IATA-DGR Class
-
Regulatory
information
UN number
Proper shipping
name
Classes
PG*
-
Not
regulated.
14 .
PG* : Packing group
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Elution Buffer for 6xHis Kit
Corrosive material
Target organ effects
HCS Classification
Regulatory information
U.S. Federal regulations
United States inventory (TSCA 8b): All components are listed or exempted.
Clean Water Act (CWA) 307: No products were found.
Clean Water Act (CWA) 311: No products were found.
Clean Air Act (CAA) 112 accidental release prevention: No products were found.
Clean Air Act (CAA) 112 regulated flammable substances: No products were found.
Clean Air Act (CAA) 112 regulated toxic substances: No products were found.
CEPA Toxic substances: None of the components are listed.
Canadian ARET: None of the components are listed.
Canadian NPRI: None of the components are listed.
Alberta Designated Substances: None of the components are listed.
Ontario Designated Substances: None of the components are listed.
Quebec Designated Substances: None of the components are listed.
Australia inventory (AICS): All components are listed or exempted.
China inventory (IECSC): All components are listed or exempted.
Korea inventory (KECI): All components are listed or exempted.
Philippines inventory (PICCS): All components are listed or exempted.
Japan inventory (ENCS): All components are listed or exempted.
WHMIS (Canada)
Class D-1B: Material causing immediate and serious toxic effects (Toxic).
Class D-2A: Material causing other toxic effects (Very toxic).
International regulations
International lists
SARA 302/304/311/312 extremely hazardous substances: No products were found.
SARA 302/304 emergency planning and notification: No products were found.
SARA 302/304/311/312 hazardous chemicals: Elution Buffer Ingredient 1
; Elution Buffer Ingredient 5
SARA 311/312 MSDS distribution - chemical inventory - hazard identification:
Elution Buffer Ingredient 1
: Immediate (acute) health hazard, Delayed (chronic) health hazard; Elution Buffer
Ingredient 5: Immediate (acute) health hazard, Delayed (chronic) health hazard
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Canada
United States
EU regulations
15 .
Canadian lists
:
Canada inventory
: Canada inventory: All components are listed or exempted.
This product is not classified according to EU legislation.
Risk phrases
:
:
Other information
Hazardous Material
Information System (U.S.A.)
3
0
0
Health
Flammability
Physical hazards
CAUSES RESPIRATORY TRACT, EYE AND SKIN BURNS. MAY BE HARMFUL IF
SWALLOWED. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN
DAMAGE.
Label requirements
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16 .
The customer is responsible for determining the PPE code for this material.
8/9
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Elution Buffer for 6xHis Kit
Other information
16 .
0
0
3
National Fire Protection
Association (U.S.A.)
Health
Special
Instability
Flammability
Notice to reader
To the best of our knowledge, the information contained herein is accurate. However, neither the above-named
supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the
information contained herein.
Final determination of suitability of any material is the sole responsibility of the user. All materials may present
unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot
guarantee that these are the only hazards that exist.
Date of printing
Date of issue
Date of previous issue
Version
5/20/2008.
5/20/2008.
1
No previous validation.
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Indicates information that has changed from previously issued version.
Full text of R-phrases
referred to in sections 2 and
3 - Europe
: R22- Harmful if swallowed.
R34- Causes burns.
R36/37/38- Irritating to eyes, respiratory system and skin.
Full text of classifications
referred to in sections 2 and
3 - Europe
: C - Corrosive
Xn - Harmful
Xi - Irritant
This product has been classified according to the hazard criteria of the CPR and the MSDS contains all the information required by the CPR.
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