Lung diseases have been a worldwide health problem, with high endemicity in developing countries. Bronchopneumonia remains the main disease of the lung. Despite advances in modern medicines, there is no effective drug available that stimulate lung function, offer protection to the lung from damage or help to regenerate pulmonary cells destroyed by disseminated bacteria. There is an urgent need for effective drugs to replace supplement those in current use. This study was embacked upon to evaluate the effect of Asplenium bulbiferum leaf extracts on nosily infected mice with streptococcus pneumoniae isolate of suspension containing 5x106 cells ul. Streptococcus pneumoniae was obtained from human specimen, identified through cultural, morphological and biochemical examination, in addition, polymerase chain reaction PCR technique was performed using universal primers to support the identification process. The antibacterial activity was carried out using agar well diffusion technique. Tube dilution technique was used to determine the minimum Inhibition Concentration MIC and Minimum Bactericidal Concentration MBC using double fold serial dilution at concentrations of 400mg ml to 25mg ml. The effect of the leaf extract was evaluated by treating mus musculus mice WBC count 4.5x103 with sub MIC of A. bulbiferum extracts after nosily infected with S. pneumoniae and appearance of disease septum with 11.3x103 WBC count after 5days from infection. The photochemical analysis revealed the presence of alkaloids phenolics, tannins, saponins, flavonoids, steroids and glycosides. The ethanolic extract exhibited more activity than the aqueous extract against S. aureus most followed by S. pneumoniae, H. influenza and K. pneumoniae was the least. After treating the infected mice with the plant extract and after 6 days from infection, the WBC count reduced to 5.1x103 l which is normal range and the mice were healthy with good physiological behaviour. The infected mice and untreated with extracts, the WBC and other immunological parameters remain high even after 16 days from infection. The study showed that Asplenium bulbiferum possessed antibacterial properties and should serve as alternative therapy for ameliorating lung infections. Eze, H. C | Ajogwu, T. M | Ozor C. N | Gloria, C. I | Soludo, O. C "The In-Vivo Effects of Asplenum Bulbiferum Ethanolic Leaf Extracts on Four Common Bacteria Associated with Lung Diseases" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd47622.pdf Paper URL : https://www.ijtsrd.com/biological-science/microbiology/47622/the-invivo-effects-of-asplenum-bulbiferum-ethanolic-leaf-extracts-on-four-common-bacteria-associated-with-lung-diseases/eze-h-c
International Journal of Trend in Scientific Research and Development (IJTSRD)
Volume 5 Issue 6, September-October 2021 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470
@ IJTSRD | Unique Paper ID – IJTSRD47622 | Volume – 5 | Issue – 6 | Sep-Oct 2021
Page 1622
The In-Vivo Effects of Asplenum Bulbiferum Ethanolic Leaf
Extracts on Four Common Bacteria Associated with Lung Diseases
Eze, H. C
1
; Ajogwu, T. M
1
; Ozor C. N
1
; Ike, Gloria Chinelo
2
; Soludo, O. C
1
1Department of Microbiology Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
2Science Laboratory Technology, Federal Polytechnic, Oko, Nigeria
ABSTRACT
Lung diseases have been a worldwide health problem, with high
endemicity in developing countries. Bronchopneumonia remains the
main disease of the lung. Despite advances in modern medicines, there
is no-effective drug available that stimulate lung function, offer
protection to the lung from damage or help to regenerate pulmonary
cells destroyed by disseminated bacteria. There is an urgent need for
effective drugs to replace/supplement those in current use. This study
was embacked upon to evaluate the effect of Asplenium bulbiferum leaf
extracts on nosily infected mice with streptococcus pneumoniae isolate
of suspension containing 5x106 cells/ul. Streptococcus pneumoniae was
obtained from human specimen, identified through cultural,
morphological and biochemical examination, in addition, polymerase
chain reaction (PCR) technique was performed using universal primers
to support the identification process. The antibacterial activity was
carried out using agar well diffusion technique. Tube dilution technique
was used to determine the minimum Inhibition Concentration (MIC)
and Minimum Bactericidal Concentration (MBC) using double fold
serial dilution at concentrations of 400mg/ml to 25mg/ml. The effect of
the leaf extract was evaluated by treating mus musculus mice (WBC
count 4.5x103) with sub-MIC of A. bulbiferum extracts after nosily
infected with S.