In USA: In Europe: Thermo Fisher Scientific Perbio Science P.O. Box 117 Industriezone III Rockford, IL 61105 Industrielaan 27 USA 9320 Erembodegem-Aalst 815.968.0747 or Belgium 1.800.874.3723 Tel:+32 53 83 44 04 Fax:+32 53 83 76 38 Conforms to 93/112/EC and ISO 11014-1 Material Safety Data Sheet Pierce® Native IgG Detection Reagent (AP) 21233 Product Name Supplier Manufacturer Product no. In case of emergency Section 1. Chemical Product and Company Identification Responsible Name MSDS_Specialist Use of the substance/preparation Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. Eng CHEMTREC: 800.424.9300 OUTSIDE US: 703.527.3887 Print date 10/15/2007 10/15/2007 Validation date MSDS# 7856 Pierce 3747 N. Meridian Road P.O. Box 117 Rockford, IL 61105 USA 815.968.0747 or 1.800.874.3723 (815)968-7316 fax www.thermo.com Substance/preparation Preparation : Section 2. Composition, Information on Ingredients CAS number Ingredient name % EC number Classification Glycerol 56-81-5 1 - 3 200-289-5 Xi; R36/37/38 Dermal contact. Eye contact. Inhalation. Ingestion. Emergency overview Medical conditions aggravated by overexposure Repeated exposure to a highly toxic material may produce general deterioration of health by an accumulation in one or many human organs. Not available. Over-exposure signs/symptoms Section 3. Hazards identification Routes of entry Carcinogenic effects Avoid contact with skin and clothing. Avoid breathing vapor or mist. Keep container closed. Use only with adequate ventilation. Wash thoroughly after handling. CARCINOGENIC EFFECTS: Classified None. by OSHA [glycerin]. MUTAGENIC EFFECTS: Classified PROVEN for human [glycerin]. Mutagenic for mammalian somatic cells. [glycerin]. TERATOGENIC EFFECTS: Not available. Review the most current and approved institutional guideline, protocol, standard operating procedure(s) and MSDS(s) for the proper handling of institutional materials/equipment associated with the use of this product. Warning! CAUSES RESPIRATORY TRACT, EYE AND SKIN IRRITATION. CONTAINS MATERIAL WHICH CAUSES DAMAGE TO THE FOLLOWING ORGANS: KIDNEYS, RESPIRATORY TRACT, SKIN, EYE, LENS OR CORNEA. United States Potential acute health effects Irritating to respiratory system. Irritating to eyes. No known significant effects or critical hazards. Irritating to skin. Eyes Skin Inhalation Ingestion Potential chronic health effects Target organs Contains material which causes damage to the following organs: kidneys, upper respiratory tract, skin, eye, lens or cornea. Continued on Next Page Pierce® Native IgG Detection Reagent (AP) Page: 2/7 Physical/chemical hazards Human health hazards Environmental hazards Not applicable. Not applicable. Not applicable. See toxicological Information (section 11) Classification Not classified. Europe Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical attention if symptoms appear. Skin contact Ingestion In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Get medical attention if irritation occurs. In case of contact, immediately flush skin with plenty of water. Remove contaminated clothing and shoes. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention. Slightly hazardous in case of inhalation (lung irritant). Not available. Slightly hazardous in case of skin contact (irritant, sensitizer). Skin inflammation is characterized by itching, scaling, reddening, or, occasionally, blistering. Skin contact Slightly hazardous in case of eye contact (irritant). Eye contact Inhalation Aggravating conditions Repeated exposure to a highly toxic material may produce general deterioration of health by an accumulation in one or many human organs. First-Aid measures If inhaled, remove to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. Get medical attention. Notes to physician Not available. Not available. Protection of first-aiders Ingestion Effects and symptoms Inhalation Eye contact Section 4. First aid measures Notice to reader Get immediate medical attention. Hazardous thermal decomposition products These products are carbon oxides (CO, CO2). May be combustible at high temperature. The lowest known value is Closed cup: 159.85°C (319.7°F). Open cup: 176°C (348.8°F). (glycerin) Use an extinguishing agent suitable for surrounding fires. Slightly flammable to flammable in presence of open flames, sparks and static discharge, of heat, of oxidizing materials. Because of the large amount of water contained in the product, the product might be combustible only after partial or complete evaporation. Section 5. Fire fighting measures Flammability of the product Flash Points Fire hazards in presence of various substances Fire fighting media and instructions Be sure to use an approved/certified respirator or equivalent. Protective clothing (fire) Absorb with an inert material and put the spilled material in an appropriate waste disposal. Finish cleaning by spreading water on the contaminated surface and allow to evacuate through the sanitary system. Section 6. Accidental release measures Small spill and leak Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal container. Finish cleaning by spreading water on the contaminated surface and dispose of according to local and regional authority requirements. Environmental precautions and clean-up methods Personal precautions Safety glasses. Lab coat. Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Gloves. Continued on Next Page Pierce® Native IgG Detection Reagent (AP) Page: 3/7 Keep container tightly closed. Keep container in a cool, well-ventilated area. Wash thoroughly after handling. Section 7. Handling and storage Handling Storage Suitable / Not suitable Packaging materials Use original container. Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. Glycerol ACGIH (United States). TWA: 10 mg/m3 ACGIH TLV (United States, 1/2007). TWA: 10 mg/m3 8 hour(s). Form: Mist TWA: 10 mg/m3 8 hour(s). Form: Total particulates OSHA PEL (United States, 11/2006). TWA: 5 mg/m3 8 hour(s). Form: Respirable fraction TWA: 15 mg/m3 8 hour(s). Form: Total dust TWA: 15 mg/m3 8 hour(s). Form: Total dust OSHA PEL (United States). Notes: Respirable TWA: 15 mg/m3 8 hour(s). OSHA PEL 1989 (United States, 3/1989). TWA: 5 mg/m3 8 hour(s). Form: Respirable fraction TWA: 10 mg/m3 8 hour(s). Form: Total dust United States Ingredient Name Occupational Exposure Limits Engineering Controls Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their respective occupational exposure limits. Ensure that eyewash stations and safety showers are proximal to the work- station location. Section 8. Exposure Controls, Personal Protection Exposure Limit Values Glycerol MAK-Werte Liste (Germany, 7/2006). Spitzenbegrenzung: 100 mg/m3 4 times per shift, 15 minute(s). Form: Aerosol / measured as the inhalable fraction TWA: 50 mg/m3 8 hour(s). Form: Aerosol / measured as the inhalable fraction Glycerol INRS (France, 6/2006). Notes: indicative exposure limits VME: 10 mg/m3 8 hour(s). Form: Aerosol France Germany Glycerol Lijst Grenswaarden / Valeurs Limites (Belgium, 3/2006). TWA: 10 mg/m3 8 hour(s). Form: mist Belgium Glycerol SUVA (Switzerland, 1/2007). Kurzzeitsgrenzwerte: 100 mg/m3 15 minute(s). Form: inhalable dust MAK: 50 mg/m3 8 hour(s). Form: inhalable dust Switzerland Glycerol EH40-WEL (United Kingdom (UK), 9/2006). TWA: 10 mg/m3 8 hour(s). Form: Mist United Kingdom (UK) Glycerol Työterveyslaitos, Sosiaali- ja terveysministeriö (Finland, 4/2005). TWA: 20 mg/m3 8 hour(s). Finland Spain Glycerol INSHT (Spain, 1/2007). VLA-ED: 10 mg/m3 8 hour(s). Form: Mist Ireland Continued on Next Page Pierce® Native IgG Detection Reagent (AP) Page: 4/7 Personal Protection Eyes Body Hands Protective Clothing (Pictograms) Gloves. Lab coat. Splash goggles. Respiratory Respirator is not needed under normal and intended conditions of use, if exposures are kept below established limits. Glycerol NAOSH (Ireland, 3/2002). OELV: 10 mg/m3 8 hour(s). Form: Mist The lowest known value is 100°C (212°F) (water). Weighted average: 105.32°C (221.6°F) Liquid. Neutral. May start to solidify at 19.85°C (67.7°F) based on data for: glycerin. Weighted average: 0.56°C (33°F) Weighted average: 1.01 (Water = 1) The highest known value is 3.1 (Air = 1) (glycerin). Weighted average: 0.69 (Air = 1) The highest known value is 2.3 kPa (17.5 mm Hg) (at 20°C) (water). Weighted average: 2.24 kPa (16.8 mm Hg) (at 20°C) See solubility in water, methanol, acetone. Easily soluble in cold water, hot water, methanol, acetone. The lowest known value is 374.2°C (705.6°F) (water). Not applicable. Characteristic. Not available. Colorless to light yellow. Boiling/condensation point Melting/freezing point 0.36 (water) compared to (n-BUTYL ACETATE=1) Dynamic: The highest known value is 1069 cP (glycerin) Weighted average: 30.92 cP Physical State Molecular weight pH Critical temperature Specific Gravity Vapor pressure Vapor density Evaporation rate Viscosity Dispersion properties Solubility Odor Taste Color Section 9. Physical and chemical properties Flash point The lowest known value is Closed cup: 159.85°C (319.7°F). Open cup: 176°C (348.8°F). (glycerin) The product is stable. Not available. Sensitive to light. (3(2h)-isothiazolone, 2-methyl-) Section 10. Stability and reactivity Stability and Reactivity Conditions to avoid Materials to avoid Hazardous Decomposition Products Not available. water: ORAL (LD50): Acute: >90000 mg/kg [ Rat]. glycerin: ORAL (LD50): Acute: 12600 mg/kg [Rat]. 4090 mg/kg [Mouse]. 7750 mg/kg [Guinea pig]. DERMAL (LD50): Acute: >10000 mg/kg [Rabbit]. 1,3-dioxane, 5-bromo-5-nitro-: ORAL (LD50): Acute: 455 mg/kg [Rat]. 590 mg/kg [Mouse]. 3(2h)-isothiazolone, 2-methyl- LD50: Not available. LC50: Not available. Section 11. Toxicological information Toxicity to Animals Continued on Next Page Pierce® Native IgG Detection Reagent (AP) Page: 5/7 Slightly hazardous in case of eye contact (irritant), of inhalation (lung irritant). CARCINOGENIC EFFECTS: Classified None. by OSHA [glycerin]. MUTAGENIC EFFECTS: Classified PROVEN for human [glycerin]. Mutagenic for mammalian somatic cells. [glycerin]. DEVELOPMENTAL TOXICITY: Classified Reproductive system/toxin/female, Reproductive system/toxin/male [SUSPECTED] [glycerin]. Contains material which causes damage to the following organs: kidneys, upper respiratory tract, skin, eye, lens or cornea. Not available. Exposure can cause nausea, headache and vomiting. (glycerin) To the best of our knowledge, the toxicological properties of this substance have not been thoroughly investigated. Chronic Effects on Humans Other Toxic Effects on Humans Special Remarks on Toxicity to Animals Special Remarks on Chronic Effects on Humans Special Remarks on Other Toxic Effects on Humans Skin Ingestion Inhalation No known significant effects or critical hazards. No known significant effects or critical hazards. No known significant effects or critical hazards. Over-exposure signs/symptoms Target organs Contains material which causes damage to the following organs: kidneys, upper respiratory tract, skin, eye, lens or cornea. No known significant effects or critical hazards. Carcinogenicity No known significant effects or critical hazards. Mutagenicity No known significant effects or critical hazards. Reproductive toxicity Ecotoxicity Not available. Mobility Germany water class Not available. Not available. Not available. Persistence/degradability Bioaccumulative potential VCI WGK: No products were found. Section 12. Ecological information Mobility Not available. : Glycerol Oncorhynchus mykiss (LC50) 96 hour(s) 54000 mg/l Species Period Result Ecotoxicity data Ingredient name LogPow BCF Persistence/degradability Bioaccumulative potential Other ecological information Other adverse effects : No known significant effects or critical hazards. Ingredient name Ingredient name BOD5 COD Ingredient name Aquatic half-life Photolysis Continued on Next Page Pierce® Native IgG Detection Reagent (AP) Page: 6/7 Not available. Waste Stream Section 13. Disposal considerations Denmark – Carcinogenic waste Not available. Not available. Sweden - thermoset plastic waste Not available. European waste catalogue (EWC) To present knowledge of the supplier, this product is not regarded as hazardous waste as defined by EU Directive 91/689/EC. Hazardous waste Austria - Waste catalogue Not available. Denmark - Waste card number Not available. Denmark - Waste group Not available. Sweden - Waste group Not available. Norway - Waste number Not available. Switzerland - Waste code Not available. The generation of waste should be avoided or minimized wherever possible. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Methods of disposal Norway - Hazardous waste To present knowledge of the supplier, this product is not regarded as hazardous waste as defined by SFT’s Directive on special waste. Contact the supplier for all information regarding the proper transportation method for this material. Section 14. Transport information U.S. Federal Regulations Irritating material Target organ effects HCS Classification Section 15. Regulatory information TSCA 8(b) inventory: 1,3-dioxane, 5-bromo-5-nitro-; 3(2h)-isothiazolone, 2-methyl-; water; glycerin Clean Water Act (CWA) 307: No products were found. Clean Water Act (CWA) 311: No products were found. Clean air act (CAA) 112 accidental release prevention: No products were found. Clean air act (CAA) 112 regulated flammable substances: No products were found. Clean air act (CAA) 112 regulated toxic substances: No products were found. SARA 302/304/311/312 extremely hazardous substances: No products were found. SARA 302/304 emergency planning and notification: No products were found. SARA 302/304/311/312 hazardous chemicals: glycerin SARA 311/312 MSDS distribution - chemical inventory - hazard identification: glycerin: Immediate (Acute) Health Hazard, Delayed (Chronic) Health Hazard This product is not classified according to the EU regulations. Label Requirements (Europe) 1 State regulations Continued on Next Page Pierce® Native IgG Detection Reagent (AP) Page: 7/7 Pennsylvania RTK: glycerin: (generic environmental hazard) Minnesota: glycerin Massachusetts RTK: glycerin State Regulations CEPA DSL: 1,3-dioxane, 5-bromo-5-nitro-; 3(2h)-isothiazolone, 2-methyl-; water; glycerin Australia (NICNAS): 1,3-dioxane, 5-bromo-5-nitro-; 3(2h)-isothiazolone, 2-methyl-; water; glycerin China: 1,3-dioxane, 5-bromo-5-nitro-; 3(2h)-isothiazolone, 2-methyl-; water; glycerin Germany water class: glycerin Japan (METI): 3(2h)-isothiazolone, 2-methyl-; water; glycerin Korea (TCCL): 1,3-dioxane, 5-bromo-5-nitro-; 3(2h)-isothiazolone, 2-methyl-; water; glycerin Philippines (RA6969): 1,3-dioxane, 5-bromo-5-nitro-; 3(2h)-isothiazolone, 2-methyl-; water; glycerin WHMIS (Canada) Class D-2A: Material causing other toxic effects (VERY TOXIC). This product is not classified according to the EU regulations. DSCL (EEC) International Regulations Not available. EINECS International Lists Pennsylvania RTK: glycerin: (generic environmental hazard) Minnesota: glycerin Massachusetts RTK: glycerin Version 0.01 Date of previous issue No Previous Validation Not available. Section 16. Other information References Validated by MSDS_Specialist on 10/15/2007. Verified by MSDS_Specialist. Printed 10/15/2007. Notice to reader To the best of our knowledge, the information contained herein is accurate. However, neither the above named supplier nor any of its subsidiaries assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist. Hazardous Material Information System (U.S.A.) National Fire Protection Association (U.S.A.) History of Document Changes Any information changes since last document version are marked with a triangle symbol. Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. Full text of classifications referred to in sections 2 and 3 - Europe Full text of R phrases referred to in sections 2 and 3 - Europe Xi - Irritant R36/37/38- Irritating to eyes, respiratory system and skin. 2 0 0 C * Health Fire hazard Reactivity Personal protection 0 0 2 Health Special Instability Flammability