Custom Baculovirus Service
1. Baculovirus Expression Full Service
20 µg of recombinant baculovirus transfer plasmid containing gene of interest.
3 cell pellets for evaluation of baculovirus to be used;
18 sets of cell pellets (including 6 negative controls) and media for
evaluation of insect host and expression conditions;
500 ml of high-titer recombinant baculovirus stock solution ready to
express the protein of interest.
and Evaluation of
Cotransfection of recombinant baculovirus transfer vector containing
gene of interest with 3 different linearized Baculovirus DNAs.
Recombinant clones will be identified and amplified. The customer will
receive 3 cell pellets derived from the amplification for evaluation. The
customer will choose the best recombinant baculovirus for the protein
Amplification of the selected recombinant baculovirus stock to a high-titer
stock of more than 1 x 108 pfu per ml. The high-titer recombinant
baculovirus stock will be used to infect three insect cell lines (Sf21, Sf9,
and T.ni) with or without booster virus and cells are harvested at two
different time points. The customer will choose the best insect host and
Amplification of High-titer
Amplification of the selected recombinant baculovirus and titer
determination of the final high-titer stock by end-point dilution. The
customer will receive 500 ml of high-titer recombinant baculovirus stock
as final product.
2. Baculovirus Expression Basic Service
20 µg of recombinant baculovirus transfer plasmid containing gene of interest