In USA: In Europe: Pierce Perbio Science P.O. Box 117 Industriezone III Rockford, IL 61105 Industrielaan 27 USA 9320 Erembodegem-Aalst 815.968.0747 or Belgium 1.800.874.3723 Tel:+32 53 83 44 04 Fax:+32 53 83 76 38 Conforms to 93/112/EC and ISO 11014-1 Material Safety Data Sheet AquaSilTM Siliconizing Fluid 442 42799 42798 42797 42799B 1873510 12/14/2006 12/14/2006 Product Name Supplier Manufacturer Product no. MSDS# Validation date Print date In case of emergency Section 1. Chemical Product and Company Identification Responsible Name MSDS Administrator Use of the substance/preparation Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. Eng Pierce Biotechnology P.O. Box 117 Rockford, IL 61105 USA 815.968.0747 or 1.800.874.3723 CHEMTREC: 800.424.9300 OUTSIDE US: 703.527.3887 Substance/preparation Preparation : Section 2. Composition, Information on Ingredients CAS number Ingredient name % EC number Classification 4-Hydroxy-4-methyl-2-pentanone 123-42-2 25 - 45 204-626-7 F; R11 Xi; R36 Tert-Butyl Alcohol 75-65-0 25 - 45 200-889-7 F; R11 Xn; R20 Xi; R36 Octadecyltrimethoxysilane 3069-42-9 10 - 20 221-339-2 Xi; R36/37/38 Dermal contact. Eye contact. Inhalation. Ingestion. Emergency overview Section 3. Hazards identification Routes of entry Avoid contact with skin and clothing. Avoid breathing vapor or mist. Keep away from heat, sparks and flame. Keep container closed. Use only with adequate ventilation. Wash thoroughly after handling. Review the most current and approved institutional guideline, protocol, standard operating procedure(s) and MSDS(s) for the proper handling of institutional materials/equipment associated with the use of this product. Danger! HIGHLY FLAMMABLE LIQUID AND VAPOR. CAUSES SEVERE EYE IRRITATION. CAUSES RESPIRATORY TRACT AND SKIN IRRITATION. CAUSES DAMAGE TO THE FOLLOWING ORGANS: LUNGS, NERVOUS SYSTEM, MUCOUS MEMBRANES, RESPIRATORY TRACT, SKIN, EYES. CONTAINS MATERIAL WHICH CAUSES DAMAGE TO THE FOLLOWING ORGANS: KIDNEYS, LIVER, BLADDER, CENTRAL NERVOUS SYSTEM, EYE, LENS OR CORNEA, THYROID. VAPOR MAY CAUSE FLASH FIRE. United States Potential acute health effects Severely irritating to the eyes. Irritating to skin. Eyes Skin Target organs Causes damage to the following organs: lungs, the nervous system, mucous membranes, upper respiratory tract, skin, eyes. Contains material which causes damage to the following organs: kidneys, liver, bladder, central nervous system (CNS), eye, lens or cornea, thyroid. Continued on Next Page AquaSilTM Siliconizing Fluid Page: 2/8 Physical/chemical hazards Human health hazards Environmental hazards Not applicable. Harmful: danger of serious damage to health by prolonged exposure through inhalation and if swallowed. Highly flammable. Medical conditions aggravated by overexposure Repeated or prolonged contact with spray mist may produce chronic eye irritation and severe skin irritation. Repeated or prolonged exposure to spray mist may produce respiratory tract irritation leading to frequent attacks of bronchial infection. Repeated exposure to a highly toxic material may produce general deterioration of health by an accumulation in one or many human organs. Not available. Over-exposure signs/symptoms Carcinogenic effects See toxicological Information (section 11) CARCINOGENIC EFFECTS: Classified None. by OSHA [AquaSilTM Siliconizing Fluid]. MUTAGENIC EFFECTS: Classified POSSIBLE for human [2-methyl-2-propanol]. Mutagenic for bacteria and/or yeast. [2-methyl-2-propanol]. TERATOGENIC EFFECTS: Classified POSSIBLE for human [2-methyl-2-propanol]. Classification F; R11 Xn; R48/20/22 Europe Irritating to respiratory system. No known significant effects or critical hazards. Inhalation Ingestion Potential chronic health effects Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical attention if symptoms appear. Skin contact Ingestion In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Get medical attention if irritation occurs. In case of contact, immediately flush skin with plenty of water. Remove contaminated clothing and shoes. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention. Hazardous in case of inhalation (lung irritant, lung corrosive). Inhalation of the spray mist may produce severe irritation of respiratory tract, characterized by coughing, choking, or shortness of breath. Over-exposure by inhalation may cause respiratory irritation. May be fatal if swallowed. May cause burns to mouth, throat and stomach. Sensitization of the product: Not available. Hazardous in case of skin contact (irritant, permeator). Corrosive to skin on contact. Skin contact may produce burns. Skin inflammation is characterized by itching, scaling, reddening, or, occasionally, blistering. Skin contact Hazardous in case of eye contact (irritant, corrosive). Eye contact Inhalation Aggravating conditions Repeated or prolonged contact with spray mist may produce chronic eye irritation and severe skin irritation. Repeated or prolonged exposure to spray mist may produce respiratory tract irritation leading to frequent attacks of bronchial infection. Repeated exposure to a highly toxic material may produce general deterioration of health by an accumulation in one or many human organs. First-Aid measures If inhaled, remove to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. Get medical attention. Notes to physician Not available. Not available. Protection of first-aiders Ingestion Effects and symptoms Inhalation Eye contact Section 4. First aid measures Notice to reader Get immediate medical attention. Flammable. Closed cup: 10°C (50°F). In case of fire, use water spray (fog), foam, dry chemicals, or CO2. Extremely flammable in presence of open flames, sparks and static discharge, of heat. Section 5. Fire fighting measures Flammability of the product Flash Points Fire hazards in presence of various substances Fire fighting media and instructions Continued on Next Page AquaSilTM Siliconizing Fluid Page: 3/8 Hazardous thermal decomposition products These products are carbon oxides (CO, CO2). Some metallic oxides. FLAMMABLE. Special Remarks on Fire Hazards Be sure to use an approved/certified respirator or equivalent. Protective clothing (fire) Keep away from heat. Keep away from sources of ignition. Stop leak if without risk. Absorb with DRY earth, sand or other non-combustible material. Do not get water inside container. Do not touch spilled material. Use water spray curtain to divert vapor drift. Prevent entry into sewers, basements or confined areas; dike if needed. Call for assistance on disposal. Section 6. Accidental release measures Small spill and leak Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal container. Environmental precautions and clean-up methods Personal precautions Safety glasses. Synthetic apron. Impervious gloves. Store in a segregated and approved area. Keep container in a cool, well-ventilated area. Keep container tightly closed and sealed until ready for use. Avoid all possible sources of ignition (spark or flame). Keep container closed. Use only with adequate ventilation. Keep away from heat, sparks and flame. To avoid fire or explosion, dissipate static electricity during transfer by grounding and bonding containers and equipment before transferring material. Use explosion-proof electrical (ventilating, lighting and material handling) equipment. Section 7. Handling and storage Handling Storage Suitable / Not suitable Packaging materials Use original container. Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. Czech Republic - Storage code I 4-Hydroxy-4-methyl-2-pentanone ACGIH (United States). TWA: 50 ppm MSHA (United States). TWA: 240 mg/m3 NIOSH (United States). TWA: 240 mg/m3 OSHA PEL (United States). TWA: 240 mg/m3 8 hour(s). Tert-Butyl Alcohol ACGIH (United States). TWA: 100 ppm OSHA PEL (United States). TWA: 300 mg/m3 8 hour(s). MSHA (United States). TWA: 300 mg/m3 NIOSH (United States). TWA: 300 mg/m3 United States Ingredient Name Occupational Exposure Limits Engineering Controls Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their respective occupational exposure limits. Ensure that eyewash stations and safety showers are proximal to the work- station location. Section 8. Exposure Controls, Personal Protection Exposure Limit Values 4-Hydroxy-4-methyl-2-pentanone KTV (Sweden). : 20 ppm NGV (Sweden). : 25 ppm Tert-Butyl Alcohol NGV: 50 ppm KTV: 75 ppm Sweden Continued on Next Page AquaSilTM Siliconizing Fluid Page: 4/8 Personal Protection Eyes Body Hands Protective Clothing (Pictograms) Gloves. Lab coat. Splash goggles. Respiratory Respirator is not needed under normal and intended conditions of use, if exposures are kept below established limits. 4-Hydroxy-4-methyl-2-pentanone MAK (Germany). : 240 mg/m3 Tert-Butyl Alcohol MAK: 100 ppm 4-Hydroxy-4-methyl-2-pentanone AN: 120 mg/m3 Tert-Butyl Alcohol AN: 25 ppm 4-Hydroxy-4-methyl-2-pentanone VME: 240 mg/m3 Tert-Butyl Alcohol VME: 100 ppm 4-Hydroxy-4-methyl-2-pentanone MAC-TGG (Netherlands). Skin : 120 mg/m3 Tert-Butyl Alcohol MAC-TGG: 100 ppm Norway France Netherlands Germany 4-Hydroxy-4-methyl-2-pentanone TWA: 240 mg/m3 Tert-Butyl Alcohol TWA: 100 ppm STEL: 150 ppm Belgium 4-Hydroxy-4-methyl-2-pentanone MAK-W (Switzerland). : 240 mg/m3 Tert-Butyl Alcohol MAK-W: 100 ppm KZG-W: 200 ppm Switzerland 4-Hydroxy-4-methyl-2-pentanone MAK (Austria). TWA: 240 mg/m3 Tert-Butyl Alcohol MAK: 50 ppm Austria Tert-Butyl Alcohol TWA: 100 ppm STEL: 150 ppm United Kingdom (UK) 4-Hydroxy-4-methyl-2-pentanone STEL: 360 mg/m3 TWA: 240 mg/m3 Finland Turkey 4-Hydroxy-4-methyl-2-pentanone NIOSH (United States). TWA: 240 mg/m3 Tert-Butyl Alcohol NIOSH (United States). TWA: 300 mg/m3 82°C (179.6°F) Liquid. May start to solidify at 25.6°C (78.1°F) based on data for: 2-methyl-2-propanol. Weighted average: -4.06°C (24.7°F) 0.9 (Water = 1) 4.6655 kPa (35 mm Hg) (at 20°C) The lowest known value is 232.9°C (451.2°F) (2-methyl-2-propanol). Not applicable. Not available. Not available. Amber. (Light.) Boiling/condensation point Melting/freezing point Physical State Molecular weight Critical temperature Specific Gravity Vapor pressure Odor Taste Color Section 9. Physical and chemical properties Continued on Next Page AquaSilTM Siliconizing Fluid Page: 5/8 >1 (Air = 1) See solubility in water, methanol, diethyl ether. Easily soluble in cold water, hot water, methanol, diethyl ether. 1.05 (2-methyl-2-propanol) compared to Butyl acetate. Vapor density Evaporation rate Dispersion properties Solubility Explosion limits Explosive properties Flash point Lower: 1% Upper: 7% Highly explosive in presence of open flames, sparks and static discharge. Closed cup: 10°C (50°F). The product is stable. Reactive with oxidizing agents, acids, alkalis, moisture. Will not occur. Not available. Section 10. Stability and reactivity Stability and Reactivity Conditions to avoid Materials to avoid Hazardous polymerization Hazardous Decomposition Products Not available. 4-Hydroxy-4-methyl-2-pentanone: ORAL (LD50): Acute: 2520 mg/kg [Rat]. 3950 mg/kg [Mouse]. DERMAL (LD50): Acute: 13500 mg/kg [Rabbit]. 2-methyl-2-propanol: ORAL (LD50): Acute: 2743 mg/kg [Rat.]. 3559 mg/kg [Rabbit]. DERMAL (LD50): Acute: >2000 mg/kg [Rabbit]. >7800 mg/kg [Guinea pig]. VAPOR (LC50): Acute: >10000 ppm 4 hour(s) [Rat]. Octadecyltrimethoxysilane LD50: Not available. LC50: Not available. Hazardous in case of eye contact (irritant, corrosive), of ingestion, of inhalation (lung irritant, lung corrosive). CARCINOGENIC EFFECTS: Classified None. by OSHA [AquaSilTM Siliconizing Fluid]. MUTAGENIC EFFECTS: Classified POSSIBLE for human [2-methyl-2-propanol]. Mutagenic for bacteria and/or yeast. [2-methyl-2-propanol]. TERATOGENIC EFFECTS: Classified POSSIBLE for human [2-methyl-2-propanol]. DEVELOPMENTAL TOXICITY: Classified Reproductive system/toxin/female [POSSIBLE] [2-methyl-2-propanol]. Causes damage to the following organs: lungs, the nervous system, mucous membranes, upper respiratory tract, skin, eyes. Contains material which causes damage to the following organs: kidneys, liver, bladder, central nervous system (CNS), eye, lens or cornea, thyroid. Not available. Material is irritating to mucous membranes and upper respiratory tract. Exposure can cause nausea, headache and vomiting. May cause convulsions. Prolonged contact can cause severe irritation or even burns. (4-Hydroxy-4-methyl- 2-pentanone) Exposure can cause stomach pains, vomiting and diarrhea. Exposure can cause coughing, chest pains, difficulty in breathing. May cause convulsions. (4-Hydroxy-4-methyl-2-pentanone) Section 11. Toxicological information Toxicity to Animals Chronic Effects on Humans Other Toxic Effects on Humans Special Remarks on Toxicity to Animals Special Remarks on Chronic Effects on Humans Special Remarks on Other Toxic Effects on Humans Skin Ingestion Inhalation Harmful: danger of serious damage to health by prolonged exposure through inhalation. No known significant effects or critical hazards. No known significant effects or critical hazards. Over-exposure signs/symptoms No known significant effects or critical hazards. Carcinogenicity No known significant effects or critical hazards. Mutagenicity No known significant effects or critical hazards. Reproductive toxicity Continued on Next Page AquaSilTM Siliconizing Fluid Page: 6/8 Target organs Causes damage to the following organs: lungs, the nervous system, mucous membranes, upper respiratory tract, skin, eyes. Contains material which causes damage to the following organs: kidneys, liver, bladder, central nervous system (CNS), eye, lens or cornea, thyroid. Ecotoxicity Not available. Mobility Germany water class Not available. Not available. Not available. Persistence/degradability Bioaccumulative potential VCI WGK: No products were found. Section 12. Ecological information Mobility Not available. : 4-Hydroxy-4-methyl-2-pentanone Bluegill. (LC50) Daphnia magna (EC50) Goldfish (LC50) 96 hour(s) 24 hour(s) 24 hour(s) 420 mg/l 9000 mg/l 5000 mg/l Tert-Butyl Alcohol Minnows (LC50) Daphnia magna (EC50) Algae (IC50) 96 hour(s) 48 hour(s) 72 hour(s) 6140 mg/l 933 mg/l >1000 mg/l Species Period Result Ecotoxicity data Ingredient name LogPow BCF Persistence/degradability Bioaccumulative potential Other ecological information Other adverse effects : No known significant effects or critical hazards. Ingredient name Ingredient name BOD5 COD Ingredient name Aquatic half-life Photolysis Biodegradability 2-methyl-2-propanol - - Readily Not available. Waste Stream Section 13. Disposal considerations Denmark – Carcinogenic waste Not available. Not available. Sweden - thermoset plastic waste Not available. European waste catalogue (EWC) The classification of the product may meet the criteria for a hazardous waste Hazardous waste Austria - Waste catalogue Not available. Denmark - Waste card number Not available. Denmark - Waste group Not available. Sweden - Waste group Not available. The generation of waste should be avoided or minimized wherever possible. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Methods of disposal Continued on Next Page AquaSilTM Siliconizing Fluid Page: 7/8 Norway - Waste number Not available. Switzerland - Waste code Not available. Norway - Hazardous waste The classification of the product may meet the criteria for a hazardous waste Contact the supplier for all information regarding the proper transportation method for this material. Section 14. Transport information U.S. Federal Regulations Flammable liquid Irritating material Target organ effects HCS Classification Section 15. Regulatory information TSCA 8(b) inventory: AquaSilTM Siliconizing Fluid Clean Water Act (CWA) 307: No products were found. Clean Water Act (CWA) 311: No products were found. Clean air act (CAA) 112 accidental release prevention: No products were found. Clean air act (CAA) 112 regulated flammable substances: No products were found. Clean air act (CAA) 112 regulated toxic substances: No products were found. CEPA DSL: 4-Hydroxy-4-methyl-2-pentanone; 2-methyl-2-propanol CEPA NDSL: Octadecyltrimethoxysilane WHMIS (Canada) Class B-2: Flammable liquid with a flash point lower than 37.8°C (100°F). Class D-2A: Material causing other toxic effects (VERY TOXIC). Class D-2B: Material causing other toxic effects (TOXIC). Class E: Corrosive liquid. International Regulations SARA 302/304/311/312 extremely hazardous substances: No products were found. SARA 302/304 emergency planning and notification: No products were found. SARA 302/304/311/312 hazardous chemicals: No products were found. SARA 311/312 MSDS distribution - chemical inventory - hazard identification: No products were found. R11- Highly flammable. R48/20/22- Harmful: danger of serious damage to health by prolonged exposure through inhalation and if swallowed. S2- Keep out of the reach of children. S46- If swallowed, seek medical advice immediately and show this container or label. Highly flammable, Harmful Label Requirements (Europe) 1 Pennsylvania RTK: 4-Hydroxy-4-methyl-2-pentanone: (generic environmental hazard); 2-methyl- 2-propanol Minnesota: 4-Hydroxy-4-methyl-2-pentanone; 2-methyl-2-propanol Massachusetts RTK: 4-Hydroxy-4-methyl-2-pentanone; 2-methyl-2-propanol New Jersey: 4-Hydroxy-4-methyl-2-pentanone; 2-methyl-2-propanol State regulations SARA 313 Form R - Reporting requirements Tert-Butyl Alcohol 75-65-0 25 - 45 : Product name CAS number Concentration SARA 313 notifications must not be detached from the MSDS and any copying and redistribution of the MSDS shall include copying and redistribution of the notice attached to copies of the MSDS subsequently redistributed. Continued on Next Page AquaSilTM Siliconizing Fluid Page: 8/8 Pennsylvania RTK: 4-Hydroxy-4-methyl-2-pentanone: (generic environmental hazard); 2-methyl-2-propanol Minnesota: 4-Hydroxy-4-methyl-2-pentanone; 2-methyl-2-propanol Massachusetts RTK: 4-Hydroxy-4-methyl-2-pentanone; 2-methyl-2-propanol New Jersey: 4-Hydroxy-4-methyl-2-pentanone; 2-methyl-2-propanol State Regulations Germany water class: 4-Hydroxy-4-methyl-2-pentanone; 2-methyl-2-propanol; Octadecyltrimethoxysilane R11- Highly flammable. R48/20/22- Harmful: danger of serious damage to health by prolonged exposure through inhalation and if swallowed. DSCL (EEC) Not available. EINECS International Lists Version 0.03 Date of previous issue 11/16/2006 Not available. Section 16. Other information References Validated by MSDS Administrator on 12/14/2006. Verified by MSDS Administrator. Printed 12/14/2006. Notice to reader To the best of our knowledge, the information contained herein is accurate. However, neither the above named supplier nor any of its subsidiaries assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist. Hazardous Material Information System (U.S.A.) National Fire Protection Association (U.S.A.) History of Document Changes Any information changes since last document version are marked with a triangle symbol. Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. Full text of classifications referred to in sections 2 and 3 - Europe Full text of R phrases referred to in sections 2 and 3 - Europe F - Highly flammable Xn - Harmful Xi - Irritant R11- Highly flammable. R20- Harmful by inhalation. R48/20/22- Harmful: danger of serious damage to health by prolonged exposure through inhalation and if swallowed. R36- Irritating to eyes. R36/37/38- Irritating to eyes, respiratory system and skin. 2 3 1 C * Health Fire hazard Reactivity Personal protection 1 3 2 Health Special Instability Flammability