Crude oil is a complex mixture of aliphatic and aromatic hydrocarbons that causes a variety of risks when released into agricultural and aquatic environment. This oil can alter population dynamics and disrupt tropic integrations and the structure of natural communities within ecosystems during spills. Microbiological and Physico chemical properties of crude oil and pristine soil samples were assessed using standard procedures. Most frequently occurring microbial isolates obtained were identified using molecular typing. Bacteria isolated from the contaminated soil were Bacillus subtilis, Bacillus cereus, Bacillus thuringiensis, Pseudomonas fluorescens, Pseudomonas aeruginosa, Alcaligenes faecalis and Klebsiella pneumoniae. Fungi isolated were Penicillium citrinnum, Aspergillus fumigatus, Aspergillus flavus and Rhizopus stolonifer. The most frequently occurring novel isolates were S. maltophilia, P. sordelli, B. thuringensis, F. solani, C. deightonni, P. citrinnum, and C. bertholletiae. Percentage organic carbon content of the crude oil contaminated soil was 21.50 while the nitrogen content was 0.90 with a pH value of 4.69. This research showed that crude oil contamination of agricultural soil distorts its physico chemical parameters which has consequent toxicity on the soil, thus possibly hampering its fitness for use in crop production. Anagboso, M. O. | Orji, M. U. | Ikele, M. O. "Microbiological and Physico-chemical Examination of Crude Oil Contaminated Soil from Awka Anambra State Nigeria" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46442.pdf Paper URL : https://www.ijtsrd.com/biological-science/microbiology/46442/microbiological-and-physicochemical-examination-of-crude-oil-contaminated-soil-from-awka-anambra-state-nigeria/anagboso-m-o
International Journal of Trend in Scientific Research and Development (IJTSRD)
Volume 5 Issue 6, September-October 2021 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470
@ IJTSRD | Unique Paper ID – IJTSRD46442 | Volume – 5 | Issue – 6 | Sep-Oct 2021
Page 635
Microbiological and Physico-chemical Examination of Crude
Oil Contaminated Soil from Awka Anambra State Nigeria
Anagboso, M. O.; Orji, M. U.; Ikele, M. O.
Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Nigeria
ABSTRACT
Crude oil is a complex mixture of aliphatic and aromatic
hydrocarbons that causes a variety of risks when released into
agricultural and aquatic environment. This oil can alter population
dynamics and disrupt tropic integrations and the structure of natural
communities within ecosystems during spills. Microbiological and
Physico-chemical properties of crude oil and pristine soil samples
were assessed using standard procedures. Most frequently occurring
microbial isolates obtained were identified using molecular typing.
Bacteria isolated from the contaminated soil were: Bacillus subtilis,
Bacillus cereus, Bacillus thuringiensis, Pseudomonas fluorescens,
Pseudomonas aeruginosa, Alcaligenes faecalis and Klebsiella
pneumoniae. Fungi isolated were Penicillium citrinnum, Aspergillus
fumigatus, Aspergillus flavus and Rhizopus stolonifer. The most
frequently occurring novel isolates were S. maltophilia, P. sordelli,
B. thuringensis, F. solani, C. deightonni, P. citrinnum, and C.
bertholletiae. Percentage organic carbon content of the crude oil
contaminated soil was 21.50% while the nitrogen content was 0.90%
with a pH value of 4.69. This research showed that crude oil
contamination of agricultural soil distorts its physico-chemical
parameters which has consequent toxicity on the soil, thus possibly
hampering its fitness for use in crop production.
KEYWORDS: Bioremediation, Crude oil and soil examination
How to cite this paper: Anagboso, M.
O. | Orji, M. U.