Gentle Ag/Ab Elution Buffer 3855 0021013 0021013B 0021027 1855170 1856282 1886380 3/11/2009. 3/11/2009. Material Safety Data Sheet Product name Manufacturer Product No. MSDS # Validation date Print date In case of emergency Product and company identification Responsible name MSDS (Regulatory Affairs) : : : : : : : 1 . CHEMTREC: 800.424.9300 OUTSIDE US: 202.483.7616 Supplier : Thermo Fisher Scientific Pierce Biotechnology P.O. Box 117 Rockford, IL 61105 United States 815.968.0747 or 800.874.3723 : Use of Substance/Preparation Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. : Thermo Fisher Scientific Pierce Biotechnology P.O. Box 117 Rockford, IL 61105 United States 815.968.0747 800.874.3723 Part of Thermo Fisher Scientific Dermal contact. Eye contact. Inhalation. Ingestion. Emergency overview Hazards identification Routes of entry Potential acute health effects Slightly irritating to the respiratory system. Slightly irritating to the eyes. No known significant effects or critical hazards. Slightly irritating to the skin. Eyes Skin Inhalation Ingestion Physical state Liquid. [Clear sparkling liquid.] MAY CAUSE RESPIRATORY TRACT, EYE AND SKIN IRRITATION. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. Slightly irritating to the eyes, skin and respiratory system. Avoid exposure - obtain special instructions before use. Do not breathe vapor or mist. Avoid contact with eyes. Avoid prolonged or repeated contact with skin. Contains material that can cause target organ damage. Use only with adequate ventilation. Keep container tightly closed and sealed until ready for use. Wash thoroughly after handling. : : : : : : : OSHA/HCS status : This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). 2 . Potential chronic health effects Carcinogenicity : No known significant effects or critical hazards. Mutagenicity : No known significant effects or critical hazards. Teratogenicity : No known significant effects or critical hazards. Developmental effects : No known significant effects or critical hazards. Chronic effects : Contains material that can cause target organ damage. 1/8 3/11/2009. Biosciences Division 3747 N. Meridian Rd. Rockford, IL (815) 968-0747 www.thermo.com/pierce Pierce PO Box 117 61105 (815) 968-7316 Fax Gentle Ag/Ab Elution Buffer Hazards identification 2 . See toxicological information (section 11) Medical conditions aggravated by over- exposure Pre-existing disorders involving any target organs mentioned in this MSDS as being at risk may be aggravated by over-exposure to this product. : Fertility effects : No known significant effects or critical hazards. Over-exposure signs/symptoms Ingestion : No specific data. Skin : Adverse symptoms may include the following: irritation redness Eyes : Adverse symptoms may include the following: irritation watering redness Inhalation : Adverse symptoms may include the following: respiratory tract irritation coughing Target organs : Contains material which causes damage to the following organs: skin, eyes. Contains material which may cause damage to the following organs: kidneys, gastrointestinal tract, central nervous system (CNS). The preparation is not classified as dangerous according to Directive 1999/45/EC and its amendments. Classification Not classified. : Magnesium Chloride Hexahydrate 7791-18-6 65 - 80 Sodium Acetate Trihydrate pHix Buffer Grade 6131-90-4 1 - 3 Composition/information on ingredients Name CAS number % United States 3 . There are no ingredients or additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section. Substance/preparation Preparation : Move exposed person to fresh air. Keep person warm and at rest. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. It may be dangerous to the person providing aid to give mouth-to- mouth resuscitation. Get medical attention. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. First aid measures Inhalation : 4 . 2/8 3/11/2009. Biosciences Division 3747 N. Meridian Rd. Rockford, IL (815) 968-0747 www.thermo.com/pierce Pierce PO Box 117 61105 (815) 968-7316 Fax Gentle Ag/Ab Elution Buffer First aid measures 4 . Wash out mouth with water. Remove dentures if any. Move exposed person to fresh air. Keep person warm and at rest. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention. Never give anything by mouth to an unconscious person. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs. Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Continue to rinse for at least 10 minutes. Get medical attention if symptoms occur. Wash clothing before reuse. Clean shoes thoroughly before reuse. Eye contact Skin contact Ingestion : : : Protection of first-aiders : No action shall be taken involving any personal risk or without suitable training. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. See section 11 for more detailed information on health effects and symptoms. Use an extinguishing agent suitable for the surrounding fire. Fire-fighting measures Extinguishing media Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training. In a fire or if heated, a pressure increase will occur and the container may burst. Special protective equipment for fire-fighters Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode. : 5 . Special exposure hazards : None known. Suitable : Not suitable : Hazardous combustion products : Decomposition products may include the following materials: carbon oxides halogenated compounds metal oxide/oxides Flammability of the product : No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Avoid breathing vapor or mist. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see section 8). Environmental precautions Accidental release measures : Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Personal precautions : 6 . 3/8 3/11/2009. Biosciences Division 3747 N. Meridian Rd. Rockford, IL (815) 968-0747 www.thermo.com/pierce Pierce PO Box 117 61105 (815) 968-7316 Fax Gentle Ag/Ab Elution Buffer Accidental release measures 6 . Stop leak if without risk. Move containers from spill area. Approach release from upwind. Prevent entry into sewers, water courses, basements or confined areas. Wash spillages into an effluent treatment plant or proceed as follows. Contain and collect spillage with non-combustible, absorbent material e.g. sand, earth, vermiculite or diatomaceous earth and place in container for disposal according to local regulations (see section 13). Dispose of via a licensed waste disposal contractor. Contaminated absorbent material may pose the same hazard as the spilled product. Note: see section 1 for emergency contact information and section 13 for waste disposal. Large spill : Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if water-soluble or absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor. Small spill : Store between the following temperatures: 2 to 8°C (35.6 to 46.4°F). Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Put on appropriate personal protective equipment (see section 8). Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Do not get in eyes or on skin or clothing. Do not breathe vapor or mist. Do not ingest. Use only with adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container. Handling and storage Handling Storage : : 7 . Exposure controls/personal protection Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator. Personal protection Respiratory Consult local authorities for acceptable exposure limits. : 8 . Engineering measures : Use only with adequate ventilation. If user operations generate dust, fumes, gas, vapor or mist, use process enclosures, local exhaust ventilation or other engineering controls to keep worker exposure to airborne contaminants below any recommended or statutory limits. Hygiene measures : Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. Recommended monitoring procedures : If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Europe No exposure limit value known. 4/8 3/11/2009. Biosciences Division 3747 N. Meridian Rd. Rockford, IL (815) 968-0747 www.thermo.com/pierce Pierce PO Box 117 61105 (815) 968-7316 Fax Gentle Ag/Ab Elution Buffer Exposure controls/personal protection 8 . Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists, gases or dusts. Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Eyes Skin : : Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Hands : Environmental exposure controls : Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. Liquid. [Clear sparkling liquid.] 6.5 to 6.6 Easily soluble in the following materials: cold water and hot water. Colorless. Physical and chemical properties Physical state pH Solubility Color : : : : 9 . The product is stable. Under normal conditions of storage and use, hazardous polymerization will not occur. No specific data. Will not occur. Under normal conditions of storage and use, hazardous decomposition products should not be produced. No specific data. Stability and reactivity Chemical stability Conditions to avoid Incompatible materials Hazardous decomposition products Possibility of hazardous reactions : : : : : 10 . Toxicological information United States 11 . Acute toxicity magnesium chloride, hexahydrate LD50 Oral Rat 8100 mg/kg - LD50 Oral Rat 7333.3 mg/kg - LDLo Intravenous Rat 176 mg/kg - Product/ingredient name Result Species Dose Exposure Conclusion/Summary : Not available. Chronic toxicity Conclusion/Summary : Not available. Carcinogenicity Conclusion/Summary : Not available. Classification Product/ingredient name ACGIH EPA NIOSH NTP IARC OSHA 5/8 3/11/2009. Biosciences Division 3747 N. Meridian Rd. Rockford, IL (815) 968-0747 www.thermo.com/pierce Pierce PO Box 117 61105 (815) 968-7316 Fax Gentle Ag/Ab Elution Buffer Toxicological information 11 . Mutagenicity magnesium chloride, hexahydrate Cytogenetic Analysis - Hamster Lung Mammalian-Animal Positive acetic acid, sodium salt, trihydrate - Mammalian-Animal Positive Product/ingredient name Test Experiment Result Conclusion/Summary : Not available. Teratogenicity Conclusion/Summary : Not available. Reproductive toxicity magnesium chloride, hexahydrate - - - None. - None. acetic acid, sodium salt, trihydrate - - - None. - - No known significant effects or critical hazards. Chronic effects : No known significant effects or critical hazards. Carcinogenicity : No known significant effects or critical hazards. Mutagenicity No known significant effects or critical hazards. Teratogenicity Developmental effects : No known significant effects or critical hazards. Fertility effects : No known significant effects or critical hazards. : : Europe Not available. Conclusion/Summary : Ecological information United States Environmental effects : No known significant effects or critical hazards. 12 . Aquatic ecotoxicity Conclusion/Summary : Not available. Other adverse effects : No known significant effects or critical hazards. The generation of waste should be avoided or minimized wherever possible. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Waste disposal Disposal considerations Disposal should be in accordance with applicable regional, national and local laws and regulations. : 13 . Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees. Hazardous waste : Within the present knowledge of the supplier, this product is not regarded as hazardous waste, as defined by EU Directive 91/689/EEC. 6/8 3/11/2009. Biosciences Division 3747 N. Meridian Rd. Rockford, IL (815) 968-0747 www.thermo.com/pierce Pierce PO Box 117 61105 (815) 968-7316 Fax Gentle Ag/Ab Elution Buffer Transport information - DOT Classification - - - Not regulated. IATA-DGR Class - Regulatory information UN number Proper shipping name Classes PG* - Not regulated. 14 . PG* : Packing group Target organ effects HCS Classification Regulatory information U.S. Federal regulations United States inventory (TSCA 8b): All components are listed or exempted. Clean Water Act (CWA) 307: No products were found. Clean Water Act (CWA) 311: No products were found. Clean Air Act (CAA) 112 accidental release prevention: No products were found. Clean Air Act (CAA) 112 regulated flammable substances: No products were found. Clean Air Act (CAA) 112 regulated toxic substances: No products were found. CEPA Toxic substances: None of the components are listed. Canadian ARET: None of the components are listed. Canadian NPRI: None of the components are listed. Alberta Designated Substances: None of the components are listed. Ontario Designated Substances: None of the components are listed. Quebec Designated Substances: None of the components are listed. Australia inventory (AICS): All components are listed or exempted. China inventory (IECSC): All components are listed or exempted. Korea inventory (KECI): All components are listed or exempted. Philippines inventory (PICCS): All components are listed or exempted. Japan inventory (ENCS): All components are listed or exempted. WHMIS (Canada) Not controlled under WHMIS (Canada). International regulations International lists SARA 302/304/311/312 extremely hazardous substances: No products were found. SARA 302/304 emergency planning and notification: No products were found. SARA 302/304/311/312 hazardous chemicals: acetic acid, sodium salt, trihydrate SARA 311/312 MSDS distribution - chemical inventory - hazard identification: acetic acid, sodium salt, trihydrate: Delayed (chronic) health hazard : : : : Canada United States EU regulations 15 . Canadian lists : Canada inventory : Canada inventory: Not determined. This product is not classified according to EU legislation. Risk phrases : : 7/8 3/11/2009. Biosciences Division 3747 N. Meridian Rd. Rockford, IL (815) 968-0747 www.thermo.com/pierce Pierce PO Box 117 61105 (815) 968-7316 Fax Gentle Ag/Ab Elution Buffer Other information Hazardous Material Information System (U.S.A.) 1 0 0 0 0 1 National Fire Protection Association (U.S.A.) Health Special Instability Flammability Health Flammability Physical hazards MAY CAUSE RESPIRATORY TRACT, EYE AND SKIN IRRITATION. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. Label requirements : Notice to reader To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist. Date of printing Date of issue Date of previous issue Version 3/11/2009. 3/11/2009. 1 No previous validation. : : : : : : 16 . The customer is responsible for determining the PPE code for this material. Indicates information that has changed from previously issued version. This product has been classified according to the hazard criteria of the CPR and the MSDS contains all the information required by the CPR. 8/8 3/11/2009. Biosciences Division 3747 N. Meridian Rd. Rockford, IL (815) 968-0747 www.thermo.com/pierce Pierce PO Box 117 61105 (815) 968-7316 Fax