In this study, clinical isolate of Staphylococcus aureus was subjected to susceptibility tests against commercially available antibiotics and Ocimum gratissimum scent leaf leaf extracts. disc diffusion nmethod was adopted for the antibiotics test while agar well diffusion technique was employed for the antimicrobial screening of the leaf extracts. The result shows that the organism was sensitive to Gentamycin, Zinnacef, Ciprofloxacin and Streptomycin representing 40 of the antibiotics while showing outright resistance to Ampiclox, Amoxicillin and Erythromycin 30 and intermediate results for the rest. Also, the cold water extract of O. gratissimum did not show any activity against the bacterium. However, both hot water extract and ethanol extract of the plant had varying degree of activity against the organism with ethanol extract recording a zone of inhibition of 25.33 mm compared with 32.00 mm recorded for Gentamycin. Furthermore, the minimum inhibitory concentration recorded ofr the extract against the organism were 10 mg ml and 2.5 mg ml respectively for hot water and ethanol extracts. These results indicate that ethanol extract of O. gratissimum leaf is very effective against Staphylococcus aureus and could be used as potential source of natural product for the treatment of infections caused by the organism especially the antibiotic resistant strains. Komolafe T. O. | Ogunyankin O. G "Antibiogram of Staphylococcus Aureus and its Sensitivity to Ocimum Gratissimum Extract" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46333.pdf Paper URL : https://www.ijtsrd.com/other-scientific-research-area/other/46333/antibiogram-of-staphylococcus-aureus-and-its-sensitivity-to-ocimum-gratissimum-extract/komolafe-t-o
International Journal of Trend in Scientific Research and Development (IJTSRD)
Volume 5 Issue 6, September-October 2021 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470
@ IJTSRD | Unique Paper ID – IJTSRD46333 | Volume – 5 | Issue – 6 | Sep-Oct 2021
Page 243
Antibiogram of Staphylococcus Aureus and its
Sensitivity to Ocimum Gratissimum Extract
Komolafe T. O., Ogunyankin O. G
Department of Science Laboratory Technology, Rufus Giwa Polytechnic, Owo, Nigeria
ABSTRACT
In this study, clinical isolate of Staphylococcus aureus was subjected
to susceptibility tests against commercially available antibiotics and
Ocimum gratissimum (scent leaf) leaf extracts. disc diffusion
nmethod was adopted for the antibiotics test while agar well diffusion
technique was employed for the antimicrobial screening of the leaf
extracts. The result shows that the organism was sensitive to
Gentamycin, Zinnacef, Ciprofloxacin and Streptomycin representing
40 % of the antibiotics while showing outright resistance to
Ampiclox, Amoxicillin and Erythromycin (30 %) and intermediate
results for the rest. Also, the cold water extract of O. gratissimum did
not show any activity against the bacterium. However, both hot water
extract and ethanol extract of the plant had varying degree of activity
against the organism with ethanol extract recording a zone of
inhibition of 25.33 mm compared with 32.00 mm recorded for
Gentamycin. Furthermore, the minimum inhibitory concentration
recorded ofr the extract against the organism were 10 mg/ml and 2.5
mg/ml respectively for hot water and ethanol extracts. These results
indicate that ethanol extract of O. gratissimum leaf is very effective
against Staphylococcus aureus and could be used as potential source
of natural product for the treatment of infections caused by the
organism especially the antibiotic resistant strains.
How to cite this paper: Komolafe T. O. |
Ogunyankin O. G "Antibiogram of
Staphylococcus Aureus
and
its
Sensitivity to Ocimum Grati